Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis. Tuberculosis lesions in children often can not cause typical clinical symptoms and imaging manifestations because of the low content of Mycobacterium tuberculosis. And for the detection of Mycobacterium tuberculosis based on Roche culture method, as the "gold standard", has long detection time and low positive rate which made it difficult for the sick children to get timely, accurate diagnosis and treatment. The primary problem for tuberculosis in children was lacking of quick and accurate diagnosis and treatment at present. Molecular diagnostic technology has been widely used and developed because of its remarkable advantages, such as rapidity, accuracy, high efficiency, easy operation etc. This article reviews the latest progress in molecular diagnostic techniques for tuberculosis in children in recent years.