哮喘患儿外周血CD4+ T细胞来源瘦素水平的变化

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (2): 122-125.

哮喘患儿外周血CD4⁺T细胞来源瘦素水平的变化

徐庆雷¹, 朱宝林¹, 马小波¹, 张巍¹刘玲玲¹, 王胜军², 陈建国²

1.沭阳县人民医院（江苏沭阳　223600）；2.江苏大学附属人民医院（江苏镇江　212002）

收稿日期:2013-08-05 出版日期:2014-02-15 发布日期:2014-02-15

The significance of peripheral blood CD4⁺ T cell-derived leptin in asthmatic children　

XU Qinglei¹, ZHU Baolin¹, MA Xiaobo¹, ZHANG Wei¹, LIU Lingling¹, WANG Shengjun²，CHEN Jianguo²

(1. Department of Clinical Laboratory, Shuyang Hospital, Shuyang 223600, Jiangsu, China; 2. The Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, Jiangsu, China)

Received:2013-08-05 Online:2014-02-15 Published:2014-02-15

摘要/Abstract

摘要：

　目的　检测哮喘患儿外周血CD4⁺T细胞瘦素表达的水平，探讨其在哮喘发生发展中的作用。方法　收集27例哮喘患儿在发作期和缓解期以及25例健康对照儿童的外周血标本，分离外周血单个核细胞（PBMC），再用CD4⁺T细胞免疫磁珠分离CD4⁺T细胞，体外培养，酶联免疫吸附试验检测上清液中瘦素水平。采用实时定量PCR方法检测PBMC中孤儿核受体γt（RORγt）相对表达量。结果　哮喘发作期、缓解期患儿以及健康对照儿童的CD4⁺T细胞培养液中瘦素水平分别为（68.46±13.08）pg/ml、（36.73±6.13）pg/ml及（32.82±5.79）pg/ml，三组间差异有统计学意义（P<0.01）；经两两比较，发作期高于缓解期和健康对照儿童，差异均有统计学意义（P<0.01）；而缓解期和健康对照儿童的差异无统计学意义（P>0.05）。哮喘发作期、缓解期患儿以及健康对照儿童的血浆瘦素水平分别为（16.64±3.53）ng/ml、（14.91±3.24）ng/ml及（13.72±5.79）ng/ml，三组间差异无统计学意义（P>0.05）。哮喘发作期、缓解期患儿以及健康对照儿童PBMC中RORγt的相对表达量分别为（0.341±0.175）、（0.089±0.028）及（0.068±0.018），三组间差异有统计学意义（P<0.01）；两两比较，发作期高于缓解期和健康对照儿童，差异均有统计学意义（P均<0.01）；缓解期和健康对照儿童的差异无统计学意义（P>0.05）。哮喘患儿发作期CD4⁺T细胞培养液中瘦素水平与PBMC中RORγt相对表达量呈正相关（r=0.681，P<0.01）。结论　哮喘患儿CD4⁺T细胞瘦素水平升高，与疾病的发生发展关系密切。

Abstract: 　Objectives　To explore the role of CD4⁺ T cell-derived leptin in peripheral blood mononuclear cells (PBMC) in asthmatic children. Methods Peripheral blood mononuclear cells were isolated from peripheral blood of both healthy subjects and asthmatic children in attack and remission stages. CD4⁺ T cells were purified from PBMCs by magnetic beads and were cultured in vitro. Supernatants were used to detect the levels of leptin by ELISA. The expression of the orphan nuclear receptor (ROR) γt was detected by real-time quantitative PCR (qRT-PCR) method. Results There was significant difference in CD4⁺ T cell-derived leptin levels of asthmatic children in attack stage (68.46±13.08 pg/ml), remission stage (36.73±6.13 pg/ml) and normal controls (32.82±5.79 pg/ml) (P<0.01). Through pairwise comparison, the leptin levels in children in attack stage were significantly higher than those in remission stage and normal control groups (P<0.01). But no statistical significance was found between remission stage group and normal controls (P>0.05). The plasma leptin of children in attack stage and remission stage, as well as in normal subjects were 16.64±3.53, 14.91±3.24 and 13.72±5.79 ng/ml respectively with no significant differences (P>0.05). The levels of RORγt mRNA were 0.341±0.175, 0.089±0.028 and 0.068±0.018 in children with asthma during attack stage, remission stage and in normal children respectively (P<0.01). Compared to remission and normal control groups, the RORγt mRNA level of children in attack stage was markedly higher (P<0.01), but there was no significant difference between asthmatic children in remission stage and the healthy controls (P>0.05). Furthermore, the result of this study showed CD4⁺T cell-derived leptin positively correlated to RORγt in asthmatic children in attack stage (r=0.681, P<0.01). Conclusions CD4⁺T cell-derived leptin is elevated in asthmatic children in attack stage and its level is closely related to the pathological process of asthma.

徐庆雷, 朱宝林, 马小波, 张巍,刘玲玲, 王胜军, 陈建国. 哮喘患儿外周血CD4⁺T细胞来源瘦素水平的变化[J]. 临床儿科杂志, 2014, 32(2): 122-125.

XU Qinglei,ZHU Baolin,MA Xiaobo,ZHANG Wei,LIU Lingling,WANG Shengjun,CHEN Jianguo. The significance of peripheral blood CD4⁺ T cell-derived leptin in asthmatic children　[J]. , 2014, 32(2): 122-125.

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