褪黑素对新生大鼠缺氧缺血后脑中galectin-3、TNF-α、IL-1β水平变化及长期行为能力的影响

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (5): 480-484.

褪黑素对新生大鼠缺氧缺血后脑中galectin-3、TNF-α、IL-1β水平变化及长期行为能力的影响

乔丽丽，沈伟勤

上海市松江区中心医院儿科（上海　201600）

收稿日期:2013-08-11 出版日期:2014-05-15 发布日期:2014-05-15

The effect of melatonin on galectin-3, TNF-α, and IL-1β in newborn rats brain damage after hypoxia-ischemia and its impact on long-term behavior　

QIAO Lili, SHEN Weiqin

(The Department of pediatrics, Songjiang Central hospital, Shanghai, China)

Received:2013-08-11 Online:2014-05-15 Published:2014-05-15

摘要/Abstract

摘要：

　目的　探讨褪黑素对新生大鼠缺氧缺血性脑损伤（HIBD）的保护作用及其机制。方法　72只5日龄Wistar大鼠随机分成生理盐水（NS）组、褪黑素组、缺氧缺血+生理盐水（HINS）组、缺氧缺血（HI）褪黑素组。各组大鼠分别在HIBD后24 h、72 h、7周处死，取脑经免疫组化染色计数半乳糖凝集素-3（galectin-3）细胞，酶联免疫吸附法测肿瘤坏死因子（TNF）-α和白介素（IL）-1β蛋白水平。HIBD后7周以恐惧测试（fear condition test）和高空十字架测试（elevated plus maze test）评估大鼠记忆能力和抗焦虑能力。结果　HIBD后72 h、7周时，HI褪黑素组皮质区的galectin-3 阳性细胞数明显少于HINS组，差异有统计学意义（P<0.05）。HIBD后24 h、72 h，HI褪黑色素组的TNF-α和IL-1β蛋白表达水平均低于HINS组，差异均有统计学意义（P<0.05）。恐惧测试中，在声音刺激前HI褪黑素组与HINS组的差异无统计学意义（P>0.05）；在声音刺激后，HI褪黑素组的恐惧百分数高于HINS组，差异有统计学意义（P<0.05）。高空十字架测试中HI褪黑素组进入开放臂的次数和停留时间百分数均少于HINS组，差异有统计学意义（P<0.05）。结论　褪黑素对HIBD后大脑损伤有短期和长期的保护作用。

Abstract: 　Objectives　To investigate neuroprotective effect of melatonin on preterm rats brain damage after hypoxia-ischemia (HI). Methods In this study, 5-day-old Wistar rats were divided into four groups: Normal saline ()group, melatonin group, HI+NS group and HI+melatonin group. HI was conducted by unilateral ligation of the left common carotid artery (ischemia) and 50 min of hypoxia. Melatonin was injected at a dose of 5mg/kg intraperitoneally three times: before ischemia, after hypoxia and 24 h after the second dose. The pups were sacrificed at 24 h, 72 h, and 7 w after HI; for galectin-3 cells count at 72 h and 7 w; TNF-α, IL-1β protein were measured in 24 h and 72 h after HI; and fear condition and elevated plus maze were tested in 7 w after HI. Results The number of galectin-3- positive cells was lower after melatonin treatment than vehicle treatment in 72 h and 7 w after HI (all P<0.05). TNF-α protein and IL-1β protein both increased at 24 h and 72 h after HI, and reduced after melatonin treatment (all P<0.05). Melatonin treatment improved memory ability and learning ability, reduced anxiety in 7 w after HI. Conclusion Melatonin has long-term and short-term protective effect on developing brain damage after HI.

乔丽丽，沈伟勤. 褪黑素对新生大鼠缺氧缺血后脑中galectin-3、TNF-α、IL-1β水平变化及长期行为能力的影响[J]. 临床儿科杂志, 2014, 32(5): 480-484.

QIAO Lili,SHEN Weiqin . The effect of melatonin on galectin-3, TNF-α, and IL-1β in newborn rats brain damage after hypoxia-ischemia and its impact on long-term behavior　[J]. , 2014, 32(5): 480-484.

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