FTO基因rs9939609位点多态性与儿童肥胖的相关性研究

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (6): 547-550.

FTO基因rs9939609位点多态性与儿童肥胖的相关性研究

吴燕明，李卫国，盛秋明，戴劲盛，张凌君，吴敏，龚惠君

上海市浦东新区人民医院儿科（上海　201200）

收稿日期:2013-10-14 出版日期:2014-06-15 发布日期:2014-06-15

Relationship between single nucleotide polymorphism on FTO gene rs9939609 and obesity in children

WU Yanming,
LI Weiguo, SHENG Qiuming, DAI Jinsheng, ZHANG Lingjun, WU Min, GONG Huijun

(Department of Pediatrics,
Shanghai Pudong New Area People’s Hospital, Shanghai 201200, China)

Received:2013-10-14 Online:2014-06-15 Published:2014-06-15

摘要/Abstract

摘要：

　目的　探讨不同程度肥胖儿童的生化指标和脂肪含量与肥胖相关基因（FTO）rs9936609单核苷酸多态性位点（SNP）之间的相关性。方法　纳入153例7~11岁儿童，其中肥胖102例、超重51例作为研究对象；160例正常体质量儿作为正常对照组。检测并比较各组间肝功能及其他生化指标的差异；利用PCR直接测序法检测FTO基因rs9939609的SNP，计算等位基因分布频率。结果　FTO基因 rs9939609 TT、TA/AA基因型在肥胖、超重和正常三组间的分布差异有统计学意义（χ²=23.01，P<0.001）；其中肥胖组和超重组TA/AA基因型表达频率均分别高于对照组，差异有统计学意义（P’<0.014）。T、A等位基因在正常对照组、超重组和肥胖组的频率分别为96.25%和3.75%，85.29%和14.71%，85.78%和14.22%，三组间的分布差异有统计学意义（χ²=21.72，P<0.001）；肥胖组和超重组A等位基因频率均分别高于对照组，差异有统计学意义（P’<0.014）。TA、AA基因型携带者体质指数（BMI）高于TT型携带者，差异有统计学意义（P<0.05）。结论　FTO基因rs9939609 多态性和儿童肥胖的发生与发展有相关性，A等位基因可导致携带者BMI升高。

Abstract: Relationship between single nucleotide polymorphism on FTO gene rs9939609 and obesity in children　WU Yanming, LI Weiguo, SHENG Qiuming, DAI Jinsheng, ZHANG Lingjun, WU Min, GONG Huijun (Department of Pediatrics, Shanghai Pudong New Area People’s Hospital, Shanghai 201200, China)#br#Objective To investigate the relationship of single nucleotide polymorphism (SNP) on FTO gene rs9939609 with metabolism index and obesity in children. Methods One hundred and fifty-three children (age 7-11 year) were recruited in this study, 102 of them were obese and 51 of them were overweight. One hundred and sixty children with normal body weight were recruited as control. Height, weight and biochemical indicator of liver function were measured. PCR and direct sequence were applied to detect the polymorphism of rs9939609, and the frequency of the allele was calculated. Results TT or TA/AA genotype frequency on FTO gene rs9939609 was significantly different among overweight group, obesity group and normal control group (χ²=23.01, P<0.001); TA/AA genotype frequency in overweight and obesity group was significant higher than that in the control group（P<0.014）. The frequencies of T and A allele in overweight group, obesity group and control group was 96.25% and 3.75%, 85.29% and 14.71%, 85.78% and 14.22% respectively. There was significant difference of allelic frequency among overweight group, obesity group and control group (χ²=21.72, P<0.001). The frequency of A allele in overweight and obesity group was higher than that in control group (P'<0.014). Subjects with TA/AA allele had significantly higher BMI compared with subjects with TT allele. Conclusions rs9939609 of FTO gene is associated with obesity in children, and allele A on this spot may raise BMI and leads to overweight and obesity.

吴燕明，李卫国，盛秋明，戴劲盛，张凌君，吴敏，龚惠君. FTO基因rs9939609位点多态性与儿童肥胖的相关性研究[J]. 临床儿科杂志, 2014, 32(6): 547-550.

WU Yanming,
LI Weiguo,SHENG Qiuming,DAI Jinsheng,ZHANG Lingjun,WU Min,GONG Huijun. Relationship between single nucleotide polymorphism on FTO gene rs9939609 and obesity in children[J]. , 2014, 32(6): 547-550.

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