›› 2014, Vol. 32 ›› Issue (1): 62-65.

• Original Article • Previous Articles     Next Articles

The effect of CD40 message activation on the renal tubular epithelial cells CHEN Yanjie2, LI Xiaozhong1, MEI Jun2, GU Li2 (1.Department of Kidney, Soochow University Affiliated Children's Hospital, Suzhou 100034, Jiangsu, China; 2.Department of Pediatrics, The Tenth People's Hospital Affiliated to Tongji University, Shanghai 200072, China)

  

  • Received:2014-01-15 Online:2014-01-15 Published:2014-01-15

Abstract:  Objective To explore the effect of CD40 message activation on the renal tubular epithelial cells (HK2). Methods After HK2 cells are activated by agonistic anti-CD40 monoclonal antibody 5c11 at 5 and 10 μg/ml, their proliferation and apoptosis were tested with MTT and flow cytometry respectively at day 3, 6 and 9. Simultaneously, level of RANTES (regulated upon activation, normal T cell expressed and secreted) in culture supernatant of HK2 cells was measured by ELISA. Results The expression of CD40 in HK2 cells was 78.9%. After activation for 9 days with 5c11 of different concentrations, the proliferation of HK2 cells (OD value) was decreased as 5c11 concentrations increased by MTT test, and the difference among groups was significant (P<0.01). However, the apoptosis rate of HK2 cells was increased at day 6 and day 9 by flow cytometry, and the difference was significant (P<0.01). The level of RANTES was elevated as 5c11 concentrations and time increased, and the differences were significant (P<0.05). Conclusions The expression of CD40 in HK2 cells plays important roles in regulating the proliferation, apoptosis and inflammatory factor release of renal tubular cells.