›› 2014, Vol. 32 ›› Issue (1): 8-11.

• Original Article • Previous Articles     Next Articles

Detection of Th17/Treg cells balance in peripheral blood in children with Henoch-Sch?nlein purpura complicated by Mycoplasma pneumoniae infection ZHANG Guohui1, WU Fuling2, LI Xiaomei2, HAN Zhaodong3, LI Ying-ying2, SHI Tao2 (1.Department of Pediatrics, Binzhou Medical University, 2.Department of Pediatrics of Affiliated Hospital, 3.Clinical Medicine Experiment Centre of Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong, China)

  

  • Received:2014-01-15 Online:2014-01-15 Published:2014-01-15

Abstract:  Objectives To investigate the role of Th17/Treg cells imbalance in the pathogenesis of Henoch-Sch?nlein purpura (HSP) complicated by Mycoplasma pneumoniae (MP) infection in children. Methods Eighteen MP-IgM-positive HSP children (HSP-MP group), 40 MP-IgM-negative HSP children (HSP group), 23 MP-IgM-positive non-HSP children (MP group) and 19 healthy children (control group) were enrolled. The percentages of Th17 and Treg cells in peripheral blood in all objects were detected by flow cytometric analysis (FCM), and serum levels of IL-17, IL-6 and TGF-β were measured by ELISA. Results The percentage of Th17 cells and the levels of IL-17 and IL-6 in HSP-MP group were significantly higher than those in other three groups (P<0.05). The percentage of Th17 cells and the levels of IL-17 and IL-6 in HSP group and MP group were significantly higher than those in control group (P<0.05). The percentage of Treg cells in HSP-MP group were significantly lower than those in other three groups (P<0.05). The percentage of Treg cells in HSP group and MP group were significantly lower than those in control group (P<0.05). There was no significant difference in the percentage of Th17 and Treg cells and the levels of IL-17 and IL-6 between HSP group and MP group (P>0.05). There was no significant difference in level of TGF-β among the four groups (P>0.05). Conclusions The imbalance of Th17/Treg cells in peripheral blood may contribute to the development of HSP complicated by MP infection in children.