Abstract: 　 Objectives　To analyze pathogen distribution and antimicrobial resistance of bacilli among children with otitis media. Methods Pathogenic bacteria was isolated from children with suppurative otitis media. The VITEK32 was used for identification. The bacterial susceptibility testing was done by Kirby-Bauer method. According to CLSI standard the antimicrobial susceptibility was determined. Results From Jan 2010 to Dec 2012, 425 children with suppurative otitis media were examined. 347 strains were isolated, of which the detectable rate was 81.65%. The detectable rate of bacteria and fungus was 93.37% (324/347) and 6.63% (23/347), respectively. Among bacteria, the detectable rate of streptococcus pneumoniae was 40.92% (142/347) and staphylococcus aureus was 33.43% (116/347). The detectable rate of haemophilus influenza was 7.78% (27/347). The prevalence of streptococcus pneumoniae is high in children aged 1-3years, with detectable rate at 47.09%. There was no statistical difference among different age groups. The prevalence of methicillin-resistant staphylococcus aureus (MRSA) in middle ear secretion was 1.11% （5/45）, 18.75% （9/48） and 30.43% （7/23） in 2010, 2011 and 2012 respectively, with no statistical difference (χ²=3.86, P=0.145). The prevalence of penicillin -resistant streptococcus pneumoniae (PRSP) in middle ear secretion was 9.26%, 3.92% and 27.03% in 2010, 2011 and 2012 respectively, with statistical difference (χ²=11.47, P=0.003). Conclusions Choosing correct therapy according to the result of middle ear secretion culture and antibiotics sensitive test can increase the recovery rate of otitismedia.