›› 2014, Vol. 32 ›› Issue (6): 540-543.

• Original Article • Previous Articles     Next Articles

Determination of alkylglycerol contents in breast milk 

QIAN Linxi1,2, SONG Huanlei1,2, ZHENG Tao5, ZHONG Yan3, YU Wenjuan4, WU Shengmei1, CAI Wei1,2   

  1. (1. Shanghai Institute for Pediatric Research, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China; 2. Shanghai Key Laboratory of Pediatric Digestion and Nutrition, Shanghai 200092, China; 3. Global Research & Discovery, Mead Johnson Nutrition, Nijmegen 6545, Netherland; 4. Analysis and Testing Center of Shanghai Jiaotong University, Shanghai 200240, China; 5. Department of Gynecology and Obstetrics, Xinhua Hospital Affilia-ted to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China)
  • Received:2013-12-19 Online:2014-06-15 Published:2014-06-15

Abstract:  Objective To determine alkylglycerol (AKG) contents and variation in breast-milk of lactating women. Methods Five cases of healthy lactating women with term delivery were selected from June 2011 to June 2012. Breast-milk samples were collected at 1, 2, 3, 4, 8, 12, 16, 20 and 24 weeks postpartum. Breast milk samples were extracted, saponificated and derivatized. AKGs composition in breast-milk was quantitatively analyzed by GC chromatography. Results Mean 16C:0 AKG content in breast-milk decreased from (17.31±3.59)×10-3g/L to (11.14±1.83)×10-3g/L. Mean 18C:0 AKG content decreased from (14.95±6.00)×10-3g/L to (9.68±2.51)×10-3g/L. Mean 18C:1 AKG content fluctuated between (4.64±0.91)×10-3g/L and (3.95±0.68)×10-3g/L. Conclusions 16C:0, 18C:0 and 18C:1 AKG contents exist in Chinese breast-milk through determination by GC chromatography, and the concentrations vary among different stages of lactation.