Abstract: 　Objective　 To investigate the interventional effect of multidimensional strategy to reduce the incidence of neonatal ventilator-associated pneumonia. Methods　The patients who were admitted to the NICU department and received mechanical ventilation (MV) for more than 48 hours from October 2012 to September 2014 were recruited. The control group received the experienced interventions from October 2012 to September 2013, neonates from October 2013 to September 2014 were recruited as the intervention group receiving multidimensional controlling strategy, including bundle care, education, process and outcome surveillance and feedback on the practices. The compliance of before-after implementation of interventions were quantitatively evaluated，and the rate of VAP was compared between the two groups. Results　The compliance rate of hand hygiene and the qualified rate of sputum suction, oral care, drain condensation from ventilator circuit, semi-recumbent position, and preventing of stress-ulcers were increased 17.0%, 11.4%, 14.7%, 18.2%, 37.5% and 56.3% respectively after implementation of multidimensional strategy, and had statistical difference (χ2=36.47-294.36，P<0.01). But the qualified rate of antibiotic use only was 66.1% in the post-VAP bundle phases, and showed no statistical difference before and after(P>0.05). The VAP rate was 41.7 cases per 1000 MV-days during control group and 19.7 cases per 1000 MV-days during intervention group, had statistical significance (P<0.05). But the rate of ventilator application showed no statistical difference between two group (P>0.05).Conclusion　The multidimensional strategy can effectively prevent the incidence of VAP.