Abstract: Objective 　To investigate the association between nucleotide polymorphisms (SNP) of rs2282430 and rs2031234 in SLC26A9 gene and clinical characteristics of asthma in Han children in central China. Methods 　A case-control study was performed. Two hundreds and three children with asthma were recruited in this study and 221 normal children were selected as controls. The genotypes of two SNPs in SLC26A9 gene were examined using PCR-RFLP. Results　Between children with asthma and controls, the distribution of three genotypes (AA, AG and GG) in rs2282430 locus had significant difference (P=0.042). The percentage of AA genotype was higher in children with asthma than that in controls. In implicit mode (AA vs. AG+GG), the two groups was statistically significant difference (P=0.028). The frequency of A allele was higher in children with asthma than that in controls (P=0.011). Between children with asthma and controls, the distribution of three genotypes (TT, GT, and GG) in rs12031234 locus had no significant difference (P=0.479). The frequency of alleles in rs12031234 locus also had no significant difference (P=0.215). Among asthmatic children with different genotype of rs2282430, the lymphocytecounts (LYM), C-reaction protein (CRP), IgE, neutrophils (NEU%), and eosinophils (EOS%) were not significantly different (P>0.05). Conclusions 　The rs2282430 polymorphism in SLC26A9 gene is associated with childhood asthma in the central China and the A allele is the risk factor. The rs2282430 polymorphism is not associated with LYM counts, CRP level, IgE level, NEU%, and EOS%.