Abstract: Objective To study the expression of hypoxia inducible factor-1 (HIF-1) and Notch signaling pathway downstream gene HES1 in the hippocampus of pubertal rats with status epilepsy (SE), and to explore the regulation site of HIF-1α in Notch signaling pathway. Methods One hundred and seventy-six 21-day-old SD rats were randomly divided into control group (NS group), pentetrazole (PTZ)-induced SE group (PTZ group), and Notch signaling pathway specific inhibitor (DAPT) intervention group (DAPT group). In PTZ group PTZ was intraperitoneally injected to build SE model and in NS group normal saline was injected as control. The intraperitoneal injection of diazepam was used to terminate SE seizures. After successful modeling, the bilateral hippocampuses were isolated after the rats were sacrificed at 0.5, 1, 2, 4 and 8 h, respectively, and RT-PCR was performed to detect the mRNA expression of HES1 and HIF-1α. The Western Blot was performed to detect protein expression in hippocampuses which were collected at 2, 4, 8, 12, and 24 h after successful modeling. DAPT group received intraperitoneal injection of DAPT 30 min before the start of molding, then the hippocampuses were isolated at 2 and 8 h after successful modeling. RT-PCR was performed to detect the mRNA expression of HES1 and HIF-1α at 2 h, and Western blot was performed to detect protein expression at 8 h. Results At each time point after SE, the expression of mRNA of HES1 and HIF-1α and the expression of protein were higher than the same time point of NS group (P < 0.05). Compared with the same time point of PTZ group, the mRNA expression of HES1 and HIF-1α and the expression of protein of DAPT group were obviously reduced (P < 0.05). Conclusion HES1 gene may be the regulatory site of HIF-1 expression in Notch signaling pathway in the hippocampus of puberty rats with SE.