Minimal residual disease (MRD) is a major cause of solid tumor relapse, which refers to the small number of malignant cells remained after therapy that cannot be detected by conventional imaging examination and morphological examination. Whereas flow cytometry and polymerase chain reaction based methods constitute the two most commonly used techniques for MRD detection. Next generation sequencing will certainly be widely employed in individual MRD detection by testing on specific genetic change in the future. This article is to review the progress on MRD detection methods in childhood solid tumor.