Objective　To summarize the application of warfarin in children. Methods　Clinical data of 362 children who used warfarin from January 2014 to December 2018 were collected and retrospectively analyzed. Results　Among the 362 patients treated with warfarin, 210 (58.0%) were male patients and 152 (42.0%) were female patients. Of them 194 (53.6%) came from department of cardiothoracic surgery and 125 (34.5%) came from Department of Cardiology. Diseases needed warfarin therapy were mainly congenital heart disease (242 cases, 66.9%) and Kawasaki disease (74 cases, 20.4%). Pediatric patients treated with warfarin were mainly from East China (262 cases, 72.4%). Conclusion　The use of warfarin in children is very different from that in adults. Children's anticoagulant therapy and research require special attention.