Objective　To explore the prevalence of cow’s milk allergy in infants in Shenzhen, China and to evaluate Cow’s milk-related Symptom Scores (CoMiSS) in predicting cow’s milk protein allergy (CMPA) in infants. Methods　A total of 210 infants with suspected CMPA (114 boys and 96 girls) with median age of 4.5 months old (1-12 months) from 6 hospitals including two municipal hospitals and four district hospitals were enrolled to calculate CoMiSS and eliminate food during June 2016 to May 2017. The 210 infants were diagnosed as CMPA or exclude CMPA by oral food challenge (OFC) test. Sensitivity and specificity of CoMiSS by ROC curve were determined, and CoMiSS in predicting CMPA in infants was evaluated as well. Results　The median CoMiSS were 7.0 (6.0~8.3), 4.0 (3.0~5.0) and 3.0 (2.0~4.0) in CMPA group, non-CMPA group and control, respectively, which showed significant differences among three groups（H=152.58, P<0.01）. Calculated by ROC curve, the area under curve of ROC was 0.90. The cut-off was 5.5. The sensitivity of CoMiSS was 87.8%, while its specificity was 78.1%. Conclusion　CoMiSS is an easy, safe tool to predict CMPA which is suitable for application in pediatric clinic and digestive clinic. It needs more multi-center studies to evaluate the criterion of integral CoMiSS≥6 in recognition of CMPA or add some scores of bloody stool.