Objective To analyze the influence factors of neonatal catheter-associated urinary tract infection (CAUTIs). Methods The clinical data of neonates with indwelling catheterization from January 2012 to February 2021 were retrospectively analyzed. According to the occurrence of CAUTIs, they were divided into CAUTIs group and non CAUTIs group and the influence factors of CAUTIs were analyzed. Results The CAUTIs occurred in 63 (17.4%) of 362 neonates. There were 38 boys and 25 girls with gestational age of 37 ( 36 - 40 ) weeks and birth weight of ( 2 . 82 ±0 . 79 ) kg. Binary logistic regression analysis showed that time and frequency of indwelling catheter were independent risk factors for CAUTIs (P