Objective To summarize the clinical characteristics of chronic spontaneous urticaria (CSU) in children. Methods The clinical data of children with CSU from January 2020 to June 2021 were retrospectively analyzed. Results A total of 698 children (356 boys and 342 girls) with CSU were enrolled, and the median age was 4.0 (2.0-7.0) years. The commonest age group was 1-7 years old (474 cases, 67.9%). The median course of disease was 6.0 (3.0-12.0) months. There were predisposing factors such as infection, food and vaccine in 119 children (17.1%) and there were 211 children (30.2%) with atopic diseases. Antinuclear antibody (ANA) test was performed in 79 patients, and 16 patients (20.3%) were positive. A total of 84 children with CSU were tested for 25-(OH) D, and the median level of 25-(OH) D was 55.1 nmol/L (26.3-80.6 nmol/L), compared with 70.2 nmol/L (55.9-93.5 nmol/L) in the control group, the difference between the two groups was statistically significant (P<0.01). Conclusion Chronic spontaneous urticaria in children has its own clinical characteristics. There is a certain proportion of positive antinuclear antibody and 25-hydroxyvitamin D deficiency, which deserves attention.