Objective To investigate the comorbidity and epidemiological characteristics of hyperactive behavior and allergic diseases in pre-school children in three communities of Pudong New Area in Shanghai. Methods A total of 1412 questionnaires were distributed to the parents of children in eight kindergartens from 4 to 7 years old in three communities from April to June 2021. The allergic disease screening questionnaire was mainly reported by parents and the contents were as follows: wheezing, continuous cough >1 month, asthma, eczema, allergic rhinitis, and a history of food allergies and immediate family allergies. Conners Parent Symptom Questionnaire was used to assess the children's hyperactive behavior. Results A total of 1302 valid questionnaires were received in this survey. The age of the children was (5.3±0.8) years, including 655 boys (50.3%) and 647 girls (49.7%). The rate of hyperactivity was 13.4%, the positive rate of asthma was 5.1%, and the positive rate of allergic rhinitis was 34.8% in preschool children. In pre-school children with hyperactive behavior, 10.9% had comorbidities of asthma and 59.4% had comorbidities of allergic rhinitis. Binary logistic regression analysis showed that male and allergic rhinitis were independent risk factors for hyperactive behavior in pre-school children (P<0.05). Conclusions The prevalence of hyperactive behavior comorbidity allergic diseases in pre-school children is high. Male and allergic rhinitis increase the risk of hyperactive behavior in pre-school children.