Effects of Astragalus membranaous on the proliferation and transforming growth factor β1 production of cardiac fibroblasts 

  • LIU Liping ,
  • YUAN Yonghua ,
  • PAN Li ,
  • HU Shaya ,
  • RUAN Tao ,
  • HE Xuehua ,
  • LIU Zhenyu ,
  • LUO Jianhong
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  • Department of Pediatric Cardiology, The First Affiliated Clinical College, Hunan Normal University, Changsha 410005, Hunan, China

Received date: 2015-03-15

  Online published: 2015-03-15

Abstract

Objective To observe the effect of Astragalus membranaous on angiotensin Ⅱ (Ang Ⅱ)-induced transforming growth factor β1 (TGF-β1) production of cardiac fibroblasts. Methods  Cardiac fibroblasts were cultured in vitro. Cells were allocated into 3 groups: control group, Astragalus membranaous groups (50, 100, 200 mg/ml), Ang II group (10-7 mol/L) and AngⅡ/Astragalus membranaous groups (50, 100, 200 mg/ml). The proliferation of each group was tested by methyl thiazolyl tetrazolium method. TGF-β1 was measured by ELISA. Results  The proliferation of cardiac fibroblasts had significant difference between each groups (F=71.84, P=0.000). The proliferation of cardiac fibroblasts with Ang II stimulation was higher than that of cells without Ang II stimulation (P<0.05). Astragalus membranaous inhibited Ang II-induced cardiac fibroblasts proliferation dose dependently (P<0.05). The TGF-β1 production had significant difference between each groups (F=786.81, P=0.000). The TGF-β1 production in AngII/astragalus membranaous groups was lower than that in Ang II group (P<0.05). The TGF-β1 production in Ang II group was the highest, and had significant difference as compared to other groups (P<0.05). Astragalus membranaous inhibited Ang II-induced TGF-β1 production dose dependently (P<0.05). Conclusions  Ang II can stimulate the proliferation of cardiac fibroblasts, and promote the TGF-β1 production. Astragalus membranaous can inhibit the proliferation of Ang II-induced cardiac fibroblasts, and reduce the TGF-β1 production of cardiac fibroblasts.

Cite this article

LIU Liping , YUAN Yonghua , PAN Li , HU Shaya , RUAN Tao , HE Xuehua , LIU Zhenyu , LUO Jianhong . Effects of Astragalus membranaous on the proliferation and transforming growth factor β1 production of cardiac fibroblasts [J]. Journal of Clinical Pediatrics, 2015 , 33(3) : 284 . DOI: 10.3969j.issn.1000-3606.2015.03.021

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