Genotyping analysis of epidemic strains of Mycoplasma pneumoniae in Suzhou area

  • SHAO Xuejun ,
  • FAN Liping ,
  •  LI Dong ,
  • SUN Huiquan ,
  • HAO Chuangli ,
  • XU Jun
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  • 1.Department of Pneumology, Soochow University Affiliated Children’s Hospital, Suzhou 215003, Jiangsu, China; 2.Department of Pediatrics, Binzhou Medical School Affiliated Hospital, Binzhou 256603, Shandong China; 3. Clinical Laboratory, Soochow University Affiliated Children’s Hospital, Suzhou 215003, Jiangsu, China

Received date: 2015-04-15

  Online published: 2015-04-15

Abstract

 Objective To analyze the genotype and variation of Mycoplasma pneumonia (MP) strains isolated from children with MP infection in Soochow area. Methods The nasopharyngeal secretions from hospitalized children with MP infection were collected during January 2012 and December 2013. The nested-multiplex PCR based on MP P1 gene was performed to detect the subtype of MP gene. Results In 313 samples, 304 (97.12 %) samples were classified as P1-I type and 8 (2.56%) samples were classified as P1-II type and one (0.32%) was V2 variant. Gene sequencing results were consistent with nested-multiple PCR results. Conclusions Nested-multiplex PCR is a reliable method for genotyping of MP P1 gene. During the study period, P1-I type was the common genotype and only one case of V2 variant was found.

Cite this article

SHAO Xuejun , FAN Liping ,  LI Dong , SUN Huiquan , HAO Chuangli , XU Jun . Genotyping analysis of epidemic strains of Mycoplasma pneumoniae in Suzhou area[J]. Journal of Clinical Pediatrics, 2015 , 33(4) : 338 . DOI: 10.3969 j.issn.1000-3606.2015.04.012

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