Objective　To investigate the association of single nucleotide polymorphism (rs2295080) in mTOR gene with the susceptibility to acute leukemia (AL) in Chinese children. Methods　A case-control study was performed by recruitment of 180 children with AL and 296 healthy children as controls. The genotype of this SNP was detected using PCR-RFLP. The data were analyzed by SPSS19.0. Results　There was a significant difference in genotypes in three groups (ALL, AML and control) (P=0.026). And the SNP was associated with AL, with G allele being higher in AL group than that in controls (OR=1.413, 95%CI: 1.050-1.901, P=0.022). In ALL group, G allele was also higher than that in healthy group (OR=1.456, 95%CI: 1.052-2.015, P=0.023). However, no significant association was observed in AML patients (P=0.302). In addition, ALL patients with GG genotype were associated with disease severity compared with patients with TT or GT genotype (OR=2.044, 95%CI: 0.569-7.341). Conclusion　The rs2295080 was associated with ALL, with G allele being a risk factor.