The influence of bone marrow mesenchymal stem cells on the injured cardiomyocytes and its relationship with Wnt/β-catenin signaling pathway

  • RUAN Tao ,
  • HE Xuehua ,
  • LIU Liping ,
  • YUAN Yonghua ,
  • PAN Li ,
  • LUO Jianhong ,
  • HU Shaya
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  • Department of Pediatric Cardiology, The First Affiliated Hospital of Hunan Normal University, Changsha 410005, Hunan, China

Received date: 2015-01-15

  Online published: 2015-01-15

Abstract

Objective To investigate the molecular mechanism of bone marrow mesenchymal stem cells (MSCs) in the treatment of injured cardiomyocytes and to explore the role of Wnt/β-catenin signaling pathway in this process. Methods After isolation, identification and culture for 72 hours, cardiomyocytes were divided into 3 groups: normal cardiomyocytes group (Group A), injured cardiomyocytes group (induced by 1 mg/L doxorubicin for 4 hours, Group B) and co-culture group (doxorubicininjured cardiomyocytes co-cultured with MSCs, Group C). After 72 hours of culture, apoptosis of cardiomyocytes was detected by flow cytometer, and the expression of β-catenin, c-Myc and p53 protein were tested by western blot. Results The apoptosis rates of cardiomyocytes in Group A, B and C were 0.74±0.65, 26.58±3.89 and 7.72±0.90, respectively, and the difference was significant (F=98.13, P<0.001). Through pairwise comparison, it was found that the apoptosis rate of cardiomyocytes in Group B was significantly higher than that in Group A and C (P<0.05). The results of western blot showed that the protein expression levels of β-catenin, c-Myc and p53 in Group B were significantly higher than those in Group C (P<0.01). However, in Group A, the protein expression of β-catenin, c-Myc and p53 could not be measured by western blot. Conclusions MSCs have significant anti-apoptotic effect on doxorubicin-induced cardiomyocyte injury and the molecular mechanism may related to the inhibition of Wnt/β-catenin signaling pathway.

Cite this article

RUAN Tao , HE Xuehua , LIU Liping , YUAN Yonghua , PAN Li , LUO Jianhong , HU Shaya . The influence of bone marrow mesenchymal stem cells on the injured cardiomyocytes and its relationship with Wnt/β-catenin signaling pathway[J]. Journal of Clinical Pediatrics, 2015 , 33(1) : 69 . DOI: 10.3969 j.issn.1000-3606.2015.01.017

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