Expression and clinical significance of miR-200a in childhood B-cell acute lymphoblastic leukemia

  • WANG Lizhong ,
  • LIU Danli ,
  • GAO Shunli ,
  • LIU Haiying
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  • Department of Pediatrics,The First Affiliated Hospital of Nanhua University,Hengyang 421001,Hunan,China

Received date: 2015-02-15

  Online published: 2015-02-15

Abstract

 Objective To explore the expression and clinical significance of microRNA-200a in childhood B-cell acute lymphoblastic leukemia (ALL). Methods Bone marrow samples were collected from 45 children with B-cell ALL and 18 children without hematology disease as control. Total RNA was acquired from bone marrow. qRT-PCR was performed to detect the expression level of miR-200a. Results The relative expression level of miR-200a in B-cell ALL group was significantly lower than that in control group (p<0.05); the expression of miR-200a in children over 10 years old was significantly lower than those in children under 10 years old (p<0.05); the expression of miR-200a in newly diagnosed samples was lower than those in those samples taken on Day 33 and at Week 12, respectively (p<0.05, p<0.01). In addition, the expression of miR-200a in low-risk group was higher than those in mid-risk and high-risk group, respectively (p<0.05). Conclusions Low level of miR-200a had a close correlation with the development and prognosis of childhood B cell ALL, which could be used as a potential target of therapy and a biomarker of childhood B cell ALL in the future.

Cite this article

WANG Lizhong , LIU Danli , GAO Shunli , LIU Haiying . Expression and clinical significance of miR-200a in childhood B-cell acute lymphoblastic leukemia[J]. Journal of Clinical Pediatrics, 2015 , 33(2) : 144 . DOI: 10.3969 j.issn.1000-3606.2015.02.011

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