Objective To explore the expression and clinical significance of microRNA-200a in childhood B-cell acute lymphoblastic leukemia (ALL). Methods Bone marrow samples were collected from 45 children with B-cell ALL and 18 children without hematology disease as control. Total RNA was acquired from bone marrow. qRT-PCR was performed to detect the expression level of miR-200a. Results The relative expression level of miR-200a in B-cell ALL group was significantly lower than that in control group (p<0.05); the expression of miR-200a in children over 10 years old was significantly lower than those in children under 10 years old (p<0.05); the expression of miR-200a in newly diagnosed samples was lower than those in those samples taken on Day 33 and at Week 12, respectively (p<0.05, p<0.01). In addition, the expression of miR-200a in low-risk group was higher than those in mid-risk and high-risk group, respectively (p<0.05). Conclusions Low level of miR-200a had a close correlation with the development and prognosis of childhood B cell ALL, which could be used as a potential target of therapy and a biomarker of childhood B cell ALL in the future.
WANG Lizhong
,
LIU Danli
,
GAO Shunli
,
LIU Haiying
. Expression and clinical significance of miR-200a in childhood B-cell acute lymphoblastic leukemia[J]. Journal of Clinical Pediatrics, 2015
, 33(2)
: 144
.
DOI: 10.3969 j.issn.1000-3606.2015.02.011