Objective　To build a more perfect serum protein fingerprint models for early diagnosis of ganglioneuroblastoma (GNB) in children. Methods　Thirty children with GNB and 30 normal control children were recruited. Serum samples were collected. Nonspecific serum protein was detected and studied by MB-WCX processing, SELDI-TOF-MS mass spectrometry system and MALDI-TOF/TOF platform. Results　Through the SELDL-TOX-MS processing, a peak at 5920 m/z protein markers, and the expression of the markers was high in GNB children (6180.6±2328), compared with normal control children (419.1±493.3), the difference was statistically significant (P<0.05); MALDI-TOF/TOF platform showed that the protein with a peak at 5920 m/z is identified as ApoC- Ⅲ . Conclusions　m/z peak of 5920 protein is suggested as specific biomarker of GNB in children, can provide significant reference for early diagnosis of ganglioneuroblastoma, and prognostic monitoring.