Detection and identification of serum biomarker for ganglioneuroblastoma in children

  • GUO Fei ,
  • ZHANG Da ,
  • JIA Jia ,
  •  YANG Yang ,
  • WANG Jiaxiang
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  • Department of Pediatric Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan, China

Received date: 2016-02-15

  Online published: 2016-02-15

Abstract

 Objective To build a more perfect serum protein fingerprint models for early diagnosis of ganglioneuroblastoma (GNB) in children. Methods Thirty children with GNB and 30 normal control children were recruited. Serum samples were collected. Nonspecific serum protein was detected and studied by MB-WCX processing, SELDI-TOF-MS mass spectrometry system and MALDI-TOF/TOF platform. Results Through the SELDL-TOX-MS processing, a peak at 5920 m/z protein markers, and the expression of the markers was high in GNB children (6180.6±2328), compared with normal control children (419.1±493.3), the difference was statistically significant (P<0.05); MALDI-TOF/TOF platform showed that the protein with a peak at 5920 m/z is identified as ApoC- Ⅲ . Conclusions m/z peak of 5920 protein is suggested as specific biomarker of GNB in children, can provide significant reference for early diagnosis of ganglioneuroblastoma, and prognostic monitoring.

Cite this article

GUO Fei , ZHANG Da , JIA Jia ,  YANG Yang , WANG Jiaxiang . Detection and identification of serum biomarker for ganglioneuroblastoma in children[J]. Journal of Clinical Pediatrics, 2016 , 34(2) : 124 . DOI: 10.3969 j.issn.1000-3606.2016.02.010

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