过敏性紫癜合并肺炎支原体感染儿童血Th17/Treg平衡性检测

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (1): 8-11.

过敏性紫癜合并肺炎支原体感染儿童血Th17/Treg平衡性检测

张国辉¹ 吴福玲² 李晓梅² 韩兆东³ 李营营² 石涛²

滨州医学院 1.儿科学教研室，2.附属医院儿科，3.临床医学实验中心（山东滨州　256603）

收稿日期:2014-01-15 出版日期:2014-01-15 发布日期:2014-01-15

Detection of Th17/Treg cells balance in peripheral blood in children with Henoch-Sch?nlein purpura complicated by Mycoplasma pneumoniae infection　ZHANG Guohui¹, WU Fuling², LI Xiaomei², HAN Zhaodong³, LI Ying-ying², SHI Tao² (1.Department of Pediatrics, Binzhou Medical University, 2.Department of Pediatrics of Affiliated Hospital, 3.Clinical Medicine Experiment Centre of Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong, China)

Received:2014-01-15 Online:2014-01-15 Published:2014-01-15

摘要/Abstract

摘要：

　目的　探讨外周血Th17/Treg失衡与儿童过敏性紫癜（HSP）合并肺炎支原体（MP）感染的关系。方法　选择18例MP-IgM阳性HSP患儿（HSP-MP组）、40例MP-IgM阴性HSP患儿（HSP组）、23例MP-IgM阳性非HSP患儿（MP组）、19例健康体检儿童（对照组），比较各组采用流式细胞术（FCM）检测的外周血Th17/Treg细胞百分率，酶联免疫吸附（ELISA）法检测的血清IL-17、IL-6、TGF-β水平。结果　HSP-MP组的外周血Th17细胞、IL-17、IL-6水平高于HSP组、MP组及对照组，HSP组及MP组亦高于对照组，差异均有统计学意义（P均<0.05）；HSP-MP组的Treg细胞水平低于HSP组、MP组及对照组，HSP组及MP组亦低于对照组，差异均有统计学意义（P均<0.05）；HSP组与MP组间差异无统计学意义（P>0.05）。各组间TGF-β水平的差异无统计学意义（P>0.05）。结论　Th17/Treg免疫失衡可能参与了MP-IgM阳性HSP的发病过程。

Abstract: 　Objectives　To investigate the role of Th17/Treg cells imbalance in the pathogenesis of Henoch-Sch?nlein purpura (HSP) complicated by Mycoplasma pneumoniae (MP) infection in children. Methods Eighteen MP-IgM-positive HSP children (HSP-MP group), 40 MP-IgM-negative HSP children (HSP group), 23 MP-IgM-positive non-HSP children (MP group) and 19 healthy children (control group) were enrolled. The percentages of Th17 and Treg cells in peripheral blood in all objects were detected by flow cytometric analysis (FCM), and serum levels of IL-17, IL-6 and TGF-β were measured by ELISA. Results The percentage of Th17 cells and the levels of IL-17 and IL-6 in HSP-MP group were significantly higher than those in other three groups (P<0.05). The percentage of Th17 cells and the levels of IL-17 and IL-6 in HSP group and MP group were significantly higher than those in control group (P<0.05). The percentage of Treg cells in HSP-MP group were significantly lower than those in other three groups (P<0.05). The percentage of Treg cells in HSP group and MP group were significantly lower than those in control group (P<0.05). There was no significant difference in the percentage of Th17 and Treg cells and the levels of IL-17 and IL-6 between HSP group and MP group (P>0.05). There was no significant difference in level of TGF-β among the four groups (P>0.05). Conclusions The imbalance of Th17/Treg cells in peripheral blood may contribute to the development of HSP complicated by MP infection in children.

张国辉吴福玲李晓梅韩兆东李营营石涛. 过敏性紫癜合并肺炎支原体感染儿童血Th17/Treg平衡性检测[J]. 临床儿科杂志, 2014, 32(1): 8-11.

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（收稿日期：2013-08-25）