细胞因子IL-6、IL-10在难治性肺炎患儿肺泡灌洗液中的变化及意义

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (4): 336-338.

细胞因子IL-6、IL-10在难治性肺炎患儿肺泡灌洗液中的变化及意义

梁坤，张慧玉，田玲，吴小磊，黄光举，杨晓莹

河北联合大学附属唐山市妇幼保健院（河北唐山　063000）

收稿日期:2013-10-14 出版日期:2014-04-15 发布日期:2014-04-15

Changes of IL-6 and IL-10 in bronchoalveolar lavage fluid in children with refractory pneumonia and its significance

LIANG Kun, ZHANG Huiyu, TIAN Ling, WU Xiaolei, HUANG Guangju, YANG Xiaoying

(Hebei United University Affi-liated Tangshan Maternity and Children Health Hospital, Tangshan 063000, Hebei, China)

Received:2013-10-14 Online:2014-04-15 Published:2014-04-15

摘要/Abstract

摘要：

　目的　探讨难治性肺炎患儿细胞因子水平变化及意义。方法　选取确诊为难治性肺炎患儿97例，根据其肺炎支原体（MP）感染情况分为MP感染组（53例）及非MP感染组（44例）；选取同期就诊的支气管异物患儿30例作为对照组。采用双抗体夹心ABC-ELISA法检测患儿肺泡灌洗液（BALF）中IL-6、IL-10水平。结果　难治性肺炎患儿BALF中IL-6、IL-10水平高于对照组，差异有统计学意义（P<0.05）；MP感染组BALF中的IL-6、IL-10水平高于非MP感染组，差异有统计学意义（P<0.05）。结论　IL-6、IL-10在难治性肺炎患儿，尤其是MP感染的难治性肺炎的免疫功能紊乱中起到一定的作用。

Abstract: Objective To explore the cytokines levels in bronchoalveolar lavage fluid (BALF) of children with refractory pneumonia and its significance. Methods A total of 97 children with refractory pneumonia were selected and then were divided into Mycoplasma pneumoniae (MP) infection group (53 cases) and non-MP infection group (44 cases) according to MP infection status. Meanwhile, 30 children with bronchial foreign body at our clinic were chosen as controls. ABC-double antibody sandwich ELISA method was used to detect interleukin (IL)-6 and IL-10 levels in BALF. Results The IL-6 and IL-10 levels in BALF of children with refractory pneumonia were significantly higher than those in the control group (P<0.05). Furthermore, the IL-6 and IL-10 levels in BALF in MP infection group were significantly higher than those in non-MP infection group (P<0.05). Conclusions The cytokines IL-6 and IL-10 play some role in immune dysfunction induced by refractory pneumonia, especially caused by MP infection.#br#Key words: refractory pneumonia; bronchoalveolar lavage fluid; interleukin-6; interleukin-10; child

梁坤，张慧玉，田玲，吴小磊，黄光举，杨晓莹. 细胞因子IL-6、IL-10在难治性肺炎患儿肺泡灌洗液中的变化及意义[J]. 临床儿科杂志, 2014, 32(4): 336-338.

LIANG Kun,ZHANG Huiyu,TIAN Ling,WU Xiaolei,HUANG Guangju,YANG Xiaoying . Changes of IL-6 and IL-10 in bronchoalveolar lavage fluid in children with refractory pneumonia and its significance[J]. , 2014, 32(4): 336-338.