谷氨酸受体在新生大鼠缺氧缺血脑损伤海马组织中的表达

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (4): 368-371.

谷氨酸受体在新生大鼠缺氧缺血脑损伤海马组织中的表达

唐晓娟¹, 孙斌¹, 王莹¹, 丁欣¹, 于论¹, 徐利晓², 冯星¹

苏州大学附属儿童医院1.新生儿科, 2.儿科研究所（江苏苏州　215003）

收稿日期:2013-06-14 出版日期:2014-04-15 发布日期:2014-04-15

The expression of glutamate receptor in hippocampus of neonatal rats with hypoxic-ischemic brain damage

TANG Xiaojuan¹, SUN Bin¹, WANG Ying¹, DING Xin¹, YU Lun¹, XU Lixiao², FENG Xing¹

(1.Department of Neonatology, Children's Hospital Affiliated to Soochow University, Suzhou 215003, Jiangsu, China; 2.Institute of Pediatrics, Children's Hospital Affiliated to Soochow University, Suzhou 215003, Jiangsu, China)

Received:2013-06-14 Online:2014-04-15 Published:2014-04-15

摘要/Abstract

摘要：

　目的　探讨Ca-A/K通道相关分子谷氨酸受体1（GluR1）及谷氨酸受体2（GluR2）在新生大鼠缺氧缺血脑损伤（HIBD）后海马组织中表达以及在自噬发生中的作用。方法　选取7日龄SD新生大鼠60只，随机分为HIBD组和假手术组，于HIBD造模后0、1、6、24、48、72 h取海马组织，采用Western blot法检测GluR2、GluR1及自噬标记蛋白LC3、Beclin-1的表达。结果　HIBD组6 h后脑组织即出现水肿，可见点状软化坏死灶。与假手术组相比，各时间点HIBD组的GluR2表达水平均下降，而GluR1、Beclin-1、LC3表达水平均增加，差异有统计学意义（P均<0.05）。HIBD组海马组织中LC3、Beclin-1、GluR1 和GluR2蛋白表达水平在HIBD造模后各个时间点间差异有统计学意义（F=10.65~701.14，P均<0.01）。GluR2蛋白表达于HIBD后1 h即开始下降，6 h下降较明显，24 h达最低点；GluR1、Beclin-1及LC3表达于6 h开始增加，24 h达高峰，48 h仍持续较高水平；上述蛋白表达均在72 h逐渐恢复。结论　Ca-A/K通道相关分子GluR2及GluR1在新生大鼠HIBD后海马组织自噬性死亡中发挥重要的作用。

Abstract: 　Objective　To study the expression of Ca-A/K channel-related molecules glutamate receptor 2 and 1(GluR2/1) in hippocampus tissues of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods A total of 60 7-day-old Sprague-Dawley rats were randomly divided into sham operation group and HIBD group. Hippocampal tissues were obtained at 0 h, 1 h, 6 h, 24 h, 48 h and 72 h after HIBD. The expression of GluR2, GluR1 and autophagy marker protein Beclin-1, LC3 were detected by Western blot assay. Results Edema and focal softening and necrosis were observed 6 h after HIBD in the brains of neonatal rats. Compared with Con group, at each time point, the expression levels of GluR2 were lower while the levels of GluR1, Beclin-1 and LC3 were higher significantly in HIBD group (P<0.05). The protein levels of LC3, Beclin-1, GluR1 and GluR2 in hippocampus tissues of HIBD group were significantly different among different time points after the establishment of HIBD model (F=10.65~701.14, P<0.01). The protein level of GluR2 was decreased from 1 h to 24 h after HIBD and reached the lowest level at 24 h. The levels of GluR1, Beclin-1 and LC3 were increased at 6 h, plateaued at 24 h and remained there until 48 h. The levels of these proteins returned back to the initial level at 72 h. Conclusions Ca-A/K channel-related molecules GluR2 and GluR1 play important roles in the autophagic cell death of hippocampus tissues in neonatal rats with hypoxic-ischemic brain damage.

唐晓娟, 孙斌, 王莹, 丁欣, 于论, 徐利晓, 冯星. 谷氨酸受体在新生大鼠缺氧缺血脑损伤海马组织中的表达[J]. 临床儿科杂志, 2014, 32(4): 368-371.

TANG Xiaojuan,SUN Bin,WANG Ying,DING Xin,YU Lun,XU Lixiao,FENG Xing . The expression of glutamate receptor in hippocampus of neonatal rats with hypoxic-ischemic brain damage [J]. , 2014, 32(4): 368-371.

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