hsa-miR-1908上游启动子区的生物信息学分析

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (4): 379-383.

hsa-miR-1908上游启动子区的生物信息学分析

1.南通大学附属医院儿科（江苏南通　226001）； 2.南京医科大学附属南京妇幼保健院医学研究中心（江苏南京　210011）； 3.南京医科大学儿科研究所（江苏南京　210029）

收稿日期:2013-12-07 出版日期:2014-04-15 发布日期:2014-04-15

Bioinformatic analysis of the hsa-miR-1908 upstream promoter region

KUANG Qian huining^1,2, LI Jingyun², JI Chenbo^2,3, GUO Xirong^2,3, NI Yuhui³, XU Meiyu¹

(1.Department of Pediatrics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China; 2.Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210011, Jiangsu, China; 3.Institue of Pediatrics, Nanjing Medical University, Nanjing 210029, Jiangsu, China)

Received:2013-12-07 Online:2014-04-15 Published:2014-04-15

摘要/Abstract

摘要：

目的利用各种生物信息学工具预测hsa-miR-1908上游启动子功能，以进一步研究其在人脂肪细胞中的转录调控机制。方法应用Ensemble数据库获取hsa-miR-1908上游启动子序列，利用多种在线相关软件预测出甲基化部位和转录因子结合部位。结果获取hsa-miR-1908上游启动子序列全长1 458 bp。miR-1908启动子序列中CpG岛位于（438～756）bp、（836～937）bp、（979～1374）bp处，CpG岛的存在会抑制miR-1908启动子的转录。miR-1908有15个转录因子的结合位点。结论 miRNA启动子相关生物信息学的研究，提高对启动子的研究效率，为进一步研究miR-1908的转录调控机制提供了重要的信息。

Abstract: Objective To predict the functions of hsa-miR-1908 promoter using various bioinformatic tools, and to provide clues for further study on transcriptional regulation mechanism of miR-1908 in human adipocytes. Methods The promoter sequence of miR-1908 was obtained from Ensemble, and then the CpG islands and transcription factor binding sites were predicted by a variety of online bioinformatic tools. Results The length of the miR-1908 promoter sequence was 1 458 bp. The CpG islands, which inhibited the transcription of miR-1908, were located at (438-756) bp, (836-937) bp and (979-1374) bp. Meanwhile, 15 transcription factor binding sites were found in the promoter sequence of miR-1908. Conclusions miRNA upstream promoter related bioinformatics can not only improve the efficiency of microRNA promoter research, but also provide further important information on transcriptional regulation of miR-1908.

. hsa-miR-1908上游启动子区的生物信息学分析[J]. 临床儿科杂志, 2014, 32(4): 379-383.

KUANG Qian huining,LI Jingyun,JI Chenbo,GUO Xirong,NI Yuhui,XU Meiyu . Bioinformatic analysis of the hsa-miR-1908 upstream promoter region[J]. , 2014, 32(4): 379-383.

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