JC病毒T抗原在儿童结直肠幼年性息肉中的表达

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (6): 536-539.

JC病毒T抗原在儿童结直肠幼年性息肉中的表达

张莉娜，潘键，金玉

南京医科大学附属南京儿童医院消化科（江苏南京　210008）

收稿日期:2013-12-12 出版日期:2014-06-15 发布日期:2014-06-15

The expression of JC virus T-antigen in colorectal juvenile polyps in children

ZHANG Lina, PAN Jian, JIN Yu

(Department of Gastroenterology, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210008, Jiangsu, China)

Received:2013-12-12 Online:2014-06-15 Published:2014-06-15

摘要/Abstract

摘要：

　目的　探讨JC病毒感染与儿童结直肠幼年性息肉之间的关系。方法　应用聚合酶链式反应（PCR）及免疫组化PV-9000法检测35例儿童结直肠幼年性息肉和23例儿童正常结直肠黏膜组织中JC病毒DNA片段及T-Ag蛋白。结果　JC病毒DNA片段在儿童结直肠幼年性息肉中的检出率为31.4%（11/35），高于正常结直肠黏膜中的检出率[8.7%（2/23）]；T-Ag蛋白在儿童结直肠幼年性息肉中的阳性表达率为22.9%（8/35），在正常结直肠黏膜中无表达；差异有统计学意义（P均<0.05）。在结直肠幼年性息肉患儿的息肉组织中，JC病毒DNA片段检出率和T-Ag蛋白阳性率与患儿年龄、性别及息肉数量、部位、大小无明显关系（P均>0.05）。结论　JC病毒感染可能与儿童结直肠幼年性息肉有关，JC病毒感染可能是儿童结直肠幼年性息肉形成的危险因素之一。

Abstract: 　Objective　To investigate the relationship between Jamestown Canyon virus (JCV) infection and colorectal juvenile polyps in children. Methods　PCR and immunohistochemical staining were performed to detect JCV DNA sequences and localize T-Ag expression in 35 colorectal juvenile polyps and 23 normal colorectal mucosa. Results　The positive rate of JCV gene sequences was 31.4% (11 of 35) in the colorectal juvenile polyps and 8.7% (2 of 23) in the normal colorectal mucosa (P<0.05). T-Ag protein was expressed in 22.9% (8 of 35) of the colorectal juvenile polyps, but none in the normal colorectal mucosa (P<0.05). Gender, age, and the number, location, and size of the colorectal juvenile polyps were not significantly different between the children with JCV-positive and JCV-negative polyps (P>0.05). Conclusions　This study suggests a positive association between JCV infection and colorectal juvenile polyps in children, indicating that JCV infection may be a risk factor for colorectal juvenile po-lyps in children.

张莉娜，潘键，金玉. JC病毒T抗原在儿童结直肠幼年性息肉中的表达[J]. 临床儿科杂志, 2014, 32(6): 536-539.

ZHANG Lina,PAN Jian,JIN Yu. The expression of JC virus T-antigen in colorectal juvenile polyps in children[J]. , 2014, 32(6): 536-539.

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