母乳中烷氧基甘油含量的测定

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (6): 540-543.

母乳中烷氧基甘油含量的测定

钱林溪^{1, 2*}, 宋还雷^{1, 2*}, 郑涛⁵, 钟燕³, 喻文娟⁴, 吴圣楣¹, 蔡威^{1, 2}

1.上海交通大学医学院附属新华医院上海市儿科医学研究所（上海　200092）；2. 上海市小儿消化营养重点实验室（上海　200092）；3. Global Research & Discovery，Mead Johnson Nutrition （Nijmegen 6545, Netherland）； 4.上海交通大学分析测试中心（上海　200240）；5.上海交通大学医学院附属新华医院妇产科（上海　200092）

收稿日期:2013-12-19 出版日期:2014-06-15 发布日期:2014-06-15

Determination of alkylglycerol contents in breast milk　

QIAN Linxi^1，2, SONG Huanlei^1，2, ZHENG Tao⁵, ZHONG Yan³, YU Wenjuan⁴, WU Shengmei¹, CAI Wei^1，2

(1. Shanghai Institute for Pediatric Research, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China; 2. Shanghai Key Laboratory of Pediatric Digestion and Nutrition, Shanghai 200092, China; 3. Global Research & Discovery, Mead Johnson Nutrition, Nijmegen 6545, Netherland; 4. Analysis and Testing Center of Shanghai Jiaotong University, Shanghai 200240, China; 5. Department of Gynecology and Obstetrics, Xinhua Hospital Affilia-ted to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China)

Received:2013-12-19 Online:2014-06-15 Published:2014-06-15

摘要/Abstract

摘要：

　目的　探讨哺乳期妇女乳汁中烷氧基甘油（AKGs）含量及其变化规律。方法　选取2011年6月至2012年6月足月单胎分娩的健康产妇5例，收集哺乳期第1、2、3、4、8、12、16、20、24周母乳用于AKGs测定。母乳样品经萃取、皂化、提取、衍生化处理后，通过气质联用色谱内标法对母乳中AKGs成分进行定量分析。结果　母乳中16C∶0 AKG含量逐渐降低，均值从（17.31±3.59）×10^-3g/L降低到（11.14±1.83）×10^-3g/L，而18C∶0 AKG均值从（14.95±6.00）×10^-3g/L下降到（9.68±2.51）×10^-3g/L，18C∶1 AKG均值在（4.64±0.91）×10^-3g/L到（3.95±0.68）×10^-3g/L之间。结论　通过气质联用色谱内标法检测到哺乳妇女母乳中存在16C∶0、18C∶0和18C∶1三种AKG，其浓度在哺乳期不同阶段有所改变。

Abstract: 　Objective　To determine alkylglycerol (AKG) contents and variation in breast-milk of lactating women. Methods Five cases of healthy lactating women with term delivery were selected from June 2011 to June 2012. Breast-milk samples were collected at 1, 2, 3, 4, 8, 12, 16, 20 and 24 weeks postpartum. Breast milk samples were extracted, saponificated and derivatized. AKGs composition in breast-milk was quantitatively analyzed by GC chromatography. Results Mean 16C:0 AKG content in breast-milk decreased from （17.31±3.59）×10^-3g/L to （11.14±1.83）×10^-3g/L. Mean 18C:0 AKG content decreased from （14.95±6.00）×10^-3g/L to （9.68±2.51）×10^-3g/L. Mean 18C:1 AKG content fluctuated between （4.64±0.91）×10^-3g/L and （3.95±0.68）×10^-3g/L. Conclusions 16C:0, 18C:0 and 18C:1 AKG contents exist in Chinese breast-milk through determination by GC chromatography, and the concentrations vary among different stages of lactation.

钱林溪, 宋还雷, 郑涛, 钟燕, 喻文娟, 吴圣楣, 蔡威. 母乳中烷氧基甘油含量的测定[J]. 临床儿科杂志, 2014, 32(6): 540-543.

QIAN Linxi,SONG Huanlei,ZHENG Tao,ZHONG Yan,YU Wenjuan,WU Shengmei,CAI Wei,. Determination of alkylglycerol contents in breast milk　[J]. , 2014, 32(6): 540-543.

参考文献

[1] 唐小红. 鲨鱼肝油中角鲨烯和烷氧基甘油分析方法的建立及应用 [D]. 华南理工大学，2011.
[2] 王林祥，王利平，陶冠军. 鲨鱼肝油中烷氧基甘油的成分分析 [J]. 无锡轻工大学学报，2004，23(2)：90-93.
[3] Bordier CG， Sellier N， Foucault AP，et al. Purification and characterization of deep sea shark Centrophorus squamosus liver oil 1-O-alkylglycerol ether lipids [J]. Lipids，1996，31(5)：521-528.
[4] Hallgren B， Niklasson A， St?llberg G，et al. On the occurrence of 1-O-alkylglycerols and 1-O-(2-methoxyalkyl)glyce-rols in human colostrum，human milk，cow's milk，sheep's milk，human red bone marrow，red cells，blood plasma and a uterine carcinoma [J]. Acta Chem Scand B，1974，28(9)：1029-1034.
[5] Larbi SO，Philp RB，Gowdey CW. The effects of alkoxygly-cerols on haematopoiesis [J]. Arch Int Pharmacodyn Ther，1968，173(1)：71-86.
[6] Wang H，Rajagopal S，Reynolds S，et al. Differentiation-promoting effect of 1-O (2 methoxy) hexadecyl glycerol in human colon cancer cells [J]. J Cell Physiol，1999，178(2)：173-178.
[7] Chepenik KP，Wykle RL. Synthesis of platelet activating factor and metabolism of related lipids in embryonic cells [J]. Biochim Biophys Acta，1992，1126(2)：192-198.
[8] Juto P. Human milk stimulates B cell function [J]. Arch Dis Child，1985，60(7)：610-613.
[9] 张继东，王延琴，邱丰，等. 动植物油脂不皂化物测定方法研究 [J]. 粮食与油脂，2011，11(1)：33-35.
[10] Jason JM，Nieburg P，Marks JS. Mortality and infectious di-sease associated with infant-feeding practices in developing countries [J]. Pediatrics，1984，74(4 Pt 2)：702-727.
[11] 杨静静，庞广昌. 母乳中免疫成分及其对婴儿免疫发育作用的研究进展 [J]. 食品科学，2006，27(10)：641-644.
[12] Zhang P，Smith R，Chapkin RS，et al. Dietary (n-3) polyunsaturated fatty acids modulate murine Th1/Th2 balance toward the Th2 pole by suppression of Th1 development [J]. J Nutr，2005，135(7)：1745-1751.
[13] Field CJ，Van Aerde JE，Robinson LE，et al. Effect of provi-ding a formula supplemented with long-chain polyunsa-turated fatty acids on immunity in full-term neonates [J]. Br J Nutr，2007，99(1)：91-99.
[14] Oh SY， Jadhav LS. Effects of dietary alkylglycerols in lactating rats on immune responses in pups [J]. Pediatr Res，1994，36(3)：300-305.