临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (6): 540-543.

• 综合报道 • 上一篇    下一篇

母乳中烷氧基甘油含量的测定

钱林溪1, 2*, 宋还雷1, 2*, 郑涛5, 钟燕3, 喻文娟4, 吴圣楣1, 蔡威1, 2   

  1. 1.上海交通大学医学院附属新华医院 上海市儿科医学研究所(上海 200092);2. 上海市小儿消化营养重点实验室(上海 200092);3. Global Research & Discovery,Mead Johnson Nutrition (Nijmegen 6545, Netherland); 4.上海交通大学分析测试中心(上海 200240);5.上海交通大学医学院附属新华医院妇产科(上海 200092)
  • 收稿日期:2013-12-19 出版日期:2014-06-15 发布日期:2014-06-15

Determination of alkylglycerol contents in breast milk 

QIAN Linxi1,2, SONG Huanlei1,2, ZHENG Tao5, ZHONG Yan3, YU Wenjuan4, WU Shengmei1, CAI Wei1,2   

  1. (1. Shanghai Institute for Pediatric Research, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China; 2. Shanghai Key Laboratory of Pediatric Digestion and Nutrition, Shanghai 200092, China; 3. Global Research & Discovery, Mead Johnson Nutrition, Nijmegen 6545, Netherland; 4. Analysis and Testing Center of Shanghai Jiaotong University, Shanghai 200240, China; 5. Department of Gynecology and Obstetrics, Xinhua Hospital Affilia-ted to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China)
  • Received:2013-12-19 Online:2014-06-15 Published:2014-06-15

摘要:

 目的 探讨哺乳期妇女乳汁中烷氧基甘油(AKGs)含量及其变化规律。方法 选取2011年6月至2012年6月足月单胎分娩的健康产妇5例,收集哺乳期第1、2、3、4、8、12、16、20、24周母乳用于AKGs测定。母乳样品经萃取、皂化、提取、衍生化处理后,通过气质联用色谱内标法对母乳中AKGs成分进行定量分析。结果 母乳中16C∶0 AKG含量逐渐降低,均值从(17.31±3.59)×10-3g/L降低到(11.14±1.83)×10-3g/L,而18C∶0 AKG均值从(14.95±6.00)×10-3g/L下降到(9.68±2.51)×10-3g/L,18C∶1 AKG均值在(4.64±0.91)×10-3g/L到(3.95±0.68)×10-3g/L之间。结论 通过气质联用色谱内标法检测到哺乳妇女母乳中存在16C∶0、18C∶0和18C∶1三种AKG,其浓度在哺乳期不同阶段有所改变。

Abstract:  Objective To determine alkylglycerol (AKG) contents and variation in breast-milk of lactating women. Methods Five cases of healthy lactating women with term delivery were selected from June 2011 to June 2012. Breast-milk samples were collected at 1, 2, 3, 4, 8, 12, 16, 20 and 24 weeks postpartum. Breast milk samples were extracted, saponificated and derivatized. AKGs composition in breast-milk was quantitatively analyzed by GC chromatography. Results Mean 16C:0 AKG content in breast-milk decreased from (17.31±3.59)×10-3g/L to (11.14±1.83)×10-3g/L. Mean 18C:0 AKG content decreased from (14.95±6.00)×10-3g/L to (9.68±2.51)×10-3g/L. Mean 18C:1 AKG content fluctuated between (4.64±0.91)×10-3g/L and (3.95±0.68)×10-3g/L. Conclusions 16C:0, 18C:0 and 18C:1 AKG contents exist in Chinese breast-milk through determination by GC chromatography, and the concentrations vary among different stages of lactation.