白细胞介素-17对系膜细胞功能的影响及其分子机制

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (2): 178-182.

白细胞介素-17对系膜细胞功能的影响及其分子机制

王莉^1,3, 李秋¹, 李翠萃¹, 阳海平¹, 王莉佳², 刘玮²

重庆医科大学附属儿童医院1.肾脏免疫科，2.儿科研究所（重庆　400014）；
3.成都市妇女儿童中心医院肾内科（四川成都　610091）

收稿日期:2013-08-29 出版日期:2014-02-15 发布日期:2014-02-15

Influence of IL-17 on mesangial cell and its molecular mechanism　

WANG Li^1,3, LI Qiu¹, LI Cuicui¹, YANG Haiping¹, WANG Lijia², LIU Wei²

(1. Department of Nephrology and Immunology, 2. The Children's Research Institution, Children's Hospital of Chongqing Medical University, Chongqing 400014, China; 3. Department of Neprology, Chengdu Women and Children's Central Hospital, Chengdu 610091, Sichuan, China)

Received:2013-08-29 Online:2014-02-15 Published:2014-02-15

摘要/Abstract

摘要：

　目的　研究白细胞介素（IL）-17对系膜细胞增殖及分泌功能的影响，并探讨其可能的分子机制。方法　四甲基偶氮唑盐比色（MTT）法检测IL-17对系膜细胞增殖的影响；酶联免疫法（ELISA）检测IL-17对系膜细胞分泌IL-1β的影响；免疫细胞化学法检测系膜细胞NF-κB的表达。结果　不同浓度IL-17在不同时间点对系膜细胞增殖的影响均无统计学意义（P>0.05）；IL-17可促进系膜细胞分泌IL-1β，且呈浓度时间依赖性，差异有统计学意义（P<0.05）；同时IL-17诱导组NF-κB的表达高于空白对照组，差异有统计学意义（P<0.05）。结论　IL-17可能部分通过NF-κB途径引起系膜细胞分泌炎症因子IL-1β，加重肾脏局部的炎症状态，导致肾脏损伤。

Abstract: 　Objectives　To investigate the effect of IL-17 on proliferation and secretion of IL-1β in the human mesangial cell line (HMCL) and explore the molecular mechanism. Methods The proliferation of HMCL induced by IL-17 was detected by MTT, the secretion of IL-1β in HMCL was measured by ELISA and the expression of NF-κB was detected by immunocytochemistry. Results IL-17 did not impact HMCL proliferation (P>0.05) but dose-and time-dependently promoted the secretion of IL-1β (P<0.05). And the expression of NF-κB induced by IL-17 increased comparing to that in the control group (P<0.05). Conclusions IL-17 may induce HMCL to secrete IL-1β via NF-κB pathway that results in kidney injury.

王莉, 李秋, 李翠萃, 阳海平, 王莉佳, 刘玮. 白细胞介素-17对系膜细胞功能的影响及其分子机制[J]. 临床儿科杂志, 2014, 32(2): 178-182.

WANG Li,LI Qiu,LI Cuicui,YANG Haiping,WANG Lijia,LIU Wei. Influence of IL-17 on mesangial cell and its molecular mechanism　[J]. , 2014, 32(2): 178-182.

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