Abstract: Objectives　To understand the relationship between early coagulation function and gestational age in premature infants, in order to provide evidence for diagnosis and treatment of premature infants. Methods　Perinatal high-risk factors (placental abruption, gestational hypertension, gestational diabetes, spontaneous premature delivery) and general status (age, gender, gestational age, delivery mode, Apgar score) of 451 single premature infants were recorded. Blood coagulation function was detected within 2 hours after birth. The premature infants were divided into three groups according to gestational age, 109 cases in early preterm neonate group, 111 cases in moderate preterm neonate group, and 231 cases in late preterm neonate group. The coagulation function were analyzed and compared among different gestational age groups. Results　There were no difference in perinatal risk factors, such as placental abruption, gestational hypertension, gestational diabetes, and spontaneous premature delivery among three groups (P>0.05). There were significant difference in various coagulation parameters among three groups (P<0.05). The levels of prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen degradation products (FDP), D-dimer (DD) tended to decrease with the gestational age increasing. The level of fibrinogen (FIB) tended to increase with the gestational age increasing. Correlation analysis showed that the levels of PT, APTT, FDP, and DD were negatively correlated with gestational age (P<0.05) and the level of FIB was positively correlated with gestational age (P<0.05). Conclusion　The coagulation function in early life in premature infants is associated with gestational age. The coagulation function of premature infants is in a changing and gradually matured process, which should be monitored dynamically.