Abstract: 　Objective　To explore the clinical feature of neonatal pertussis. Methods　The clinical data of 29 neonates with pertussis admitted during 2012 to 2016 were retrospectively analyzed. Results　In 29 neonates only 1 had a history of exposure to pertussis. All of the cases had paroxysmal cough (100.0%), 22 cases had vomit after cough (75.9%), 16 cases had spasmodic cough with cyanosis (55.2%), 7 cases had crow-like echo (24.1%), and 4 cases had apnea (13.8%). 15 cases had positive deep sputum culture, and totally 16 strains of pathogenic bacteria were isolated. In addition, 4 cases were combined with respiratory syncytial virus infection. All of the patients were treated with azithromycin or clindamycin. 19 cases were recovered, 8 cases improved, and 2 cases abandoned treatment. Conclusions　Neonatal pertussis infection is occult, and usually has no clear contact history of pertussis. The clinical feature was mainly paroxysmal cough. The crow like echo was seldom. Pertussis cannot be completely excluded in neonates with positive respiratory syncytial virus.