微小残留病（MRD）是实体瘤复发的主要原因，MRD目前常用检测方法包括流式细胞术、聚合酶链反应和荧光原位 杂交技术等，新的高通量检测技术包括下一代测序等被用于发现更特异的基因改变作为检测分子标志，以进一步对MRD进行个 体化监测。目前各实体瘤MRD检测技术均各有优势及不足，文章就儿童常见的实体瘤的检测方法及进展进行综述。

　Minimal residual disease (MRD) is a major cause of solid tumor relapse, which refers to the small number of malignant cells remained after therapy that cannot be detected by conventional imaging examination and morphological examination. Whereas flow cytometry and polymerase chain reaction based methods constitute the two most commonly used techniques for MRD detection. Next generation sequencing will certainly be widely employed in individual MRD detection by testing on specific genetic change in the future. This article is to review the progress on MRD detection methods in childhood solid tumor.