目的　探讨Hedgehog信号通路相关分子BMI1和Gli1在儿童肾母细胞瘤（NB）组织中的表达情况及其与预 后的关系。方法　收集37例NB患儿的NB组织及其癌旁正常组织的病理标本，通过免疫组织化学染色法观察BMI1和 Gli1蛋白在肿瘤组织中的表达，分析其与NB患儿临床病理特征以及血清肿瘤标志物的关系。利用公共数据库分析BMI1 和Gli1 mRNA表达与NB患儿预后的关系。结果　37例患儿中男14例、女23例，中位年龄2岁（3个月~6岁）。NB组织中 BMI1和Gli1蛋白表达均较癌旁正常组织增高，差异有统计学意义（P<0.01）。BMI1阳性细胞在37例患儿肿瘤组织均被 观察到，而Gli1阳性细胞在16例患儿肿瘤组织中未检测到。22例BMI1高表达的NB患儿更容易出现远隔转移，血清甲 胎蛋白、糖类抗原-125（CA-125）表达增高，同时总体生存期也更短，差异均有统计学意义（P<0.05）；Gli1表达增高则与 NB患儿临床病理特征或预后无相关性。结论　Hedgehog通路相关分子BMI1和Gli1在NB肿瘤组织中表达增高，BMI1 与肿瘤转移和预后不良呈正相关，可能是NB发展的一个重要影响因素。

Objective　To investigate the expression of Hedgehog signaling pathway related molecules BMI1 and Gli1 in children with nephroblastoma (NB) and their relationship with prognosis. Methods　Pathological specimens of NB tissues and adjacent normal tissues in 37 NB children were collected. The expression of BMI1 and Gli1 proteins in tumor tissues was observed by immunohistochemical staining, and the relationship of BMI1 and Gli1 protein expressions with clinicopathological characteristics and serum tumor markers in NB children were analyzed. The relationship between BMI1 and Gli1 expressions and NB prognosis in children were analyzed using a public database. Results　Among the 37 children, 14 were male and 23 were female, and the median age was 2 years (3 months to 6 years). The expressions of BMI1 and Gli1 in NB tissues were higher than those in normal tissues adjacent to cancer (P<0.01). BMI1-positive cells were observed in the tumor tissues of 37 children, while Gli1 positive cells were not detected in the tumor tissues of 16 children. Twenty-two NB children with high BMI1 expression were more likely to have distant metastasis and had the increased expression of serum alpha-fetoprotein and carbohydrate antigen-125 (CA-125), and the overall survival period was shorter. The differences were statistically significant (P<0.05). Increased Gli1 expression was not associated with clinicopathological features or prognosis in NB children. Conclusion　The expression of Hedgehog pathway related molecules BMI1 and Gli1 was increased in NB tumor tissues. BMI1 is positively correlated with tumor metastasis and poor prognosis and it may be an important factor in the development of NB.