目的　分析新生儿败血症病原菌及细菌耐药情况。方法　回顾性分析2004年1月至2013年12月收治的新生儿败血症的血培养病原菌分布及药敏结果，比较前后5年的变化。结果　前后5年间，无乳链球菌检出构成比由1.0%上升至4.2%，真菌由1.9%上升到7.9%，但新生儿血培养各细菌检出分布的差异无统计学意义(P=0.282)；大肠埃希菌的超广谱β-内酰胺酶(ESBLs)发生概率由28.6%升至36.0%，但差异无统计学意义(P>0.05)，未发现对亚胺培南、美罗培南耐药大肠埃希菌；葡萄球菌对苯唑西林和氨苄西林舒巴坦的耐药性升高，差异有统计学意义(P均<0.05)，未发现耐万古霉素的葡萄球菌。结论　新生儿败血症的病原菌仍以凝固酶阴性葡萄球菌和大肠埃希菌为主；真菌和无乳链球菌的感染率有升高趋势。

Objective??To analyze distribution and antibiotic resistance pathogenic bacteria in neonatal sepsis in 10 years. Methods The distribution of pathogens and their antibiotic resistance were retrospectively analyzed in neonatal sepsis from January, 2004 to December, 2013. The results were compared between 2004-2008 and 2009-2013. Results The percentage of Streptococcus agalactiae rose from 1.0% to 4.2% and fungi rose from 1.9% to 7.9% in all pathogens in past 10 years. But the distribution of pathogenic bacteria was not significantly different. The ESBLs of Escherichia coli were increased from 28.6% to 36.0% (P>0.05). The Escherichia coli resistant to imipenem and meropenem had not been found. The Staphylococcus resistance to oxacallin and ampicillin/sulbactam was increased. The Staphylococcus resistant to vancomycin had not been found. Conclusions The main pathogens of neonatal sepsis are coagulase-negative staphylococci and E. coli. The fungi and Streptococcus agalactiae infections are significantly increased.