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信号转导与转录活化因子3 在儿童颞叶局灶性皮层发育不良IIIa 型中的表达

  • 娄燕 ,
  • 武江 ,
  • 李文玲 ,
  • 王江娅
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  • 1. 河北医科大学研究生学院( 河北石家庄 050017);2. 河北省人民医院( 河北石家庄 050051)

收稿日期: 2014-09-15

  网络出版日期: 2014-09-15

Expression of STAT3 in children with focal cortical dysplasias type IIIa

  • LOU Yan ,
  • WU JIANG ,
  • LI Wenling ,
  • WANG Jiangya
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  • 1.Hebei Medical University, Shijiazhuang 050017, Hebei, China; 2. Hebei General Hospital, Shijiazhuang 050051, Hebei, China

Received date: 2014-09-15

  Online published: 2014-09-15

摘要

目的 探讨信号转导与转录活化因子3(STAT3)在儿童难治性癫痫相关性局灶性皮层发育不良(focal cortical dysplasias,FCD)IIIa型中的表达情况。方法 选取26例FCD IIIa型患儿的颞叶皮层组织和5例颅内减压或死亡患儿的颞叶组织,分别通过免疫组化、免疫荧光和蛋白免疫印迹方法,检测并比较脑组织中STAT3、P-STAT3(ser727)的表达水平。结果 P-STAT3(ser727)在FCDIIIa患儿颞叶皮层中表达较对照组增加,两组间差异有统计学意义(P<0.01);两张连续切片分别做P-STAT3(ser727)的DAB显色和GFAP免疫荧光,证实P-STAT3阳性细胞为星形胶质细胞。结论 活化的STAT3可能参与FCDIIIa型的发病机制,并推测可能通过介导星形胶质细胞增生途径实现。

本文引用格式

娄燕 , 武江 , 李文玲 , 王江娅 . 信号转导与转录活化因子3 在儿童颞叶局灶性皮层发育不良IIIa 型中的表达[J]. 临床儿科杂志, 2014 , 32(9) : 859 . DOI: 10.3969 j.issn.1000-3606.2014.09.015

Abstract

 Objectives To detect the expression of STAT3 in children with FCD (focal cortical dysplasias) type IIIa. Methods The expression of STAT3 and P-STAT3 (ser727) were determined in temporal lobe specimens from 26 children with FCD type IIIa and from 5 normal control by immunohistochemistry, immunofluorescence and Western blot. Results The expression of P-STAT3 (ser727) was obviously higher in FCD type IIIa than in control group. P-STAT3 (ser727) was located mainly in the nucleus of astrocytes. Conclusions Activated STAT3 might contribute to the patholgenesis of FCD type IIIa by mediating proliferation of astrocytes.
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