实验研究

过敏性紫癜患儿外周血单个核细胞细胞因子信号转导抑制蛋白1、3 mRNA 的表达

  • 王凤英 ,
  • 鲁曼
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  • 1. 泰兴市人民医院儿科( 江苏泰兴 225400);2. 泰安市中心医院儿科( 山东泰安 271000)

收稿日期: 2015-01-15

  网络出版日期: 2015-01-15

Expression of suppressor of cytokine signaling 1, 3 mRNA in peripheral blood mononuclear cells in children with Henoch-Schöenlein purpura

  • WANG Fengying ,
  • LU Man
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  • 1.Department of Pediatrics, Taixing People’s Hospital, Taixing 225400, Jiangsu, China; 2.Department of Pediatrics, The Central Hospital of Taian, Taian 271000, Shandong, China

Received date: 2015-01-15

  Online published: 2015-01-15

摘要

 目的 探讨细胞因子信号转导抑制蛋白1(SOCS1)和SOCS3在儿童过敏性紫癜中的表达情况和作用。方法 应用逆转录—聚合酶链反应检测20例过敏性紫癜患儿和15例正常健康对照儿童的外周血单个核细胞中SOCS1/SOCS3mRNA表达水平。结果 过敏性紫癜患儿SOCS1、SOCS3 mRNA相对表达水平分别为(1.37±0.38)、(1.87±0.53),高于对照儿童的(0.85±0.12)、(0.77±0.13),差异均有统计学意义(P均<0.001);SOCS3 mRNA表达增高更为明显。结论 过敏性紫癜患儿有 SOCS3的高表达。

本文引用格式

王凤英 , 鲁曼 . 过敏性紫癜患儿外周血单个核细胞细胞因子信号转导抑制蛋白1、3 mRNA 的表达[J]. 临床儿科杂志, 2015 , 33(1) : 60 . DOI: 10.3969 j.issn.1000-3606.2015.01.015

Abstract

 Objective To explore the expression and role of suppressor of cytokine signaling 1 (SOCS1) and SOCS3 mRNA in the development of Henoch-Schöenlein purpura (HSP) in children. Methods Real-Time reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression levels of SOCS1 and SOCS3 mRNA in peripheral blood mononuclear cells (PBMCs) in children with HSP (HSP group, n=20) and healthy children (control group, n=15), respectively. Results The expression levels of SOCS1 and SOCS3 mRNA in HSP group were (1.37±0.38) and (1.87±0.53), and in control group were (0.85±0.12) and (0.77±0.13). Compared with control group, the expressions of SOCS1 and SOCS3 mRNA, especially the latter, were significantly up-regulated in HSP group (P<0.001). Conclusions The up-regulated expression of SOCS3 may be invol ed in the pathogenesis of HSP in children.
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