实验研究

黄芪对心肌成纤维细胞增殖及分泌转化生长因子-β1的影响#br#

  • 刘丽萍 ,
  • 袁勇华 ,
  • 潘丽 ,
  • 胡沙雅 ,
  • 阮滔 ,
  • 何学华 ,
  • 刘震宇 ,
  • 罗建红
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  • 湖南师范大学第一附属医院小儿心血管科(湖南长沙 410005)

收稿日期: 2015-03-15

  网络出版日期: 2015-03-15

基金资助

湖南省教育厅科研基金资助项目(No.13C522);湖南省中医药科研计划项目(No.2014105)

Effects of Astragalus membranaous on the proliferation and transforming growth factor β1 production of cardiac fibroblasts 

  • LIU Liping ,
  • YUAN Yonghua ,
  • PAN Li ,
  • HU Shaya ,
  • RUAN Tao ,
  • HE Xuehua ,
  • LIU Zhenyu ,
  • LUO Jianhong
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  • Department of Pediatric Cardiology, The First Affiliated Clinical College, Hunan Normal University, Changsha 410005, Hunan, China

Received date: 2015-03-15

  Online published: 2015-03-15

摘要

目的 观察黄芪注射液对血管紧张素Ⅱ(AngⅡ)诱导的心肌成纤维细胞分泌转化生长因子(TGF-β1)的影响。方法 体外培养乳鼠心脏成纤维细胞并制成单细胞悬液,分为对照组,黄芪低、中、高剂量组,AngⅡ组,AngⅡ联合黄芪低、中、高剂量组,分别加入50、100、200 mg/ml黄芪注射液,以及10-7 mol/L AngⅡ,共同培育2 d。用四甲基偶氮哇盐法测定各组细胞增殖情况,ELISA法测定培养上清TGF-β1分泌情况。结果 各组间细胞增殖率差异有统计学意义(F=71.84,P=0.000);AngⅡ组的细胞增殖率明显升高,与其余各组比较均有统计学意义(P均<0.05);AngⅡ联合黄芪后,随着黄芪剂量的逐渐增加,细胞增殖率逐渐下降,差异均有统计学意义(P<0.05)。各组间TGF-β1分泌量差异也有统计学意义(F=786.81,P=0.000);黄芪低、中、高剂量组的TGF-β1分泌量均低于对照组,差异有统计学意义(P均<0.05);AngⅡ组的TGF-β1分泌量为各组中最高,与其余各组比较均有统计学意义(P均<0.05);AngⅡ联合黄芪后,随着黄芪剂量的逐渐增加,TGF-β1分泌量逐渐下降,差异均有统计学意义(P<0.05)。结论 AngⅡ可刺激心肌成纤维细胞增殖,促进心肌成纤维细胞分泌TGF-β1,而黄芪注射液可明显抑制AngⅡ诱导心肌成纤维细胞的增殖作用,并且减少心肌成纤维细胞分泌TGF-β1。

本文引用格式

刘丽萍 , 袁勇华 , 潘丽 , 胡沙雅 , 阮滔 , 何学华 , 刘震宇 , 罗建红 . 黄芪对心肌成纤维细胞增殖及分泌转化生长因子-β1的影响#br#[J]. 临床儿科杂志, 2015 , 33(3) : 284 . DOI: 10.3969j.issn.1000-3606.2015.03.021

Abstract

Objective To observe the effect of Astragalus membranaous on angiotensin Ⅱ (Ang Ⅱ)-induced transforming growth factor β1 (TGF-β1) production of cardiac fibroblasts. Methods  Cardiac fibroblasts were cultured in vitro. Cells were allocated into 3 groups: control group, Astragalus membranaous groups (50, 100, 200 mg/ml), Ang II group (10-7 mol/L) and AngⅡ/Astragalus membranaous groups (50, 100, 200 mg/ml). The proliferation of each group was tested by methyl thiazolyl tetrazolium method. TGF-β1 was measured by ELISA. Results  The proliferation of cardiac fibroblasts had significant difference between each groups (F=71.84, P=0.000). The proliferation of cardiac fibroblasts with Ang II stimulation was higher than that of cells without Ang II stimulation (P<0.05). Astragalus membranaous inhibited Ang II-induced cardiac fibroblasts proliferation dose dependently (P<0.05). The TGF-β1 production had significant difference between each groups (F=786.81, P=0.000). The TGF-β1 production in AngII/astragalus membranaous groups was lower than that in Ang II group (P<0.05). The TGF-β1 production in Ang II group was the highest, and had significant difference as compared to other groups (P<0.05). Astragalus membranaous inhibited Ang II-induced TGF-β1 production dose dependently (P<0.05). Conclusions  Ang II can stimulate the proliferation of cardiac fibroblasts, and promote the TGF-β1 production. Astragalus membranaous can inhibit the proliferation of Ang II-induced cardiac fibroblasts, and reduce the TGF-β1 production of cardiac fibroblasts.
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