实验研究

miR-125a-5P 在髓母细胞瘤中的异常表达及其抑癌作用

  • 楚冬梅 ,
  • 李艳霞 ,
  • 高心静 ,
  • 刘晓智 ,
  • 严艳萍 ,
  • 刘翠苹 ,
  • 姜忠敏 ,
  • 姚玲
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  • 天津市第五中心医院(天津 300450)

收稿日期: 2015-11-15

  网络出版日期: 2015-11-15

基金资助

天津市滨海新区医药卫生科研基金项目(No.2012BWKZ003)

Abnormal expression of miR-125a-5P in medulloblastoma and its role as tumor suppressor

  • CHU Dongmei ,
  • LI Yanxia ,
  • GAO Xinjing ,
  • LIU Xiaozhi ,
  • YAN Yanping ,
  • LIU Cuiping ,
  • JIANG Zhongmin ,
  • YAO Ling
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  • The Fifth Central Hospital of Tianjin, Tianjin 300450, China

Received date: 2015-11-15

  Online published: 2015-11-15

摘要

目的 探讨miR-125a-5P在髓母细胞瘤中的表达特点并分析其抑癌作用。方法 采用逆转录PCR方法检测58份髓母细胞瘤组织及2个髓母细胞瘤株D341细胞和Daoy细胞中miR-125a-5p表达水平;瞬时转染D341细胞miR-125a-5P寡核苷酸,免疫荧光方法检测Ki-67核增殖活性,Transwell实验检测细胞迁移力,克隆形成实验检测瘤细胞致瘤潜能;荷瘤鼠模型检测miR-125a-5p转染对体内瘤细胞的抑制效果。结果 miR-125a-5P在儿童髓母细胞瘤组织中的相对表达量明显高于正常儿童脑组织,差异有统计学意义(t=17.55,P<0.01);miR-125a-5P在神经干细胞、髓母细胞瘤株D341细胞、Daoy细胞、神经胶质细胞和神经元细胞的相对表达量差异有统计学意义(F=9.44,P=0.002);miR-125a-5P的表达量在神经胶质细胞和神经元细胞中较高,分别高于神经干细胞、髓母细胞瘤株D341细胞和Daoy细胞中的相对表达量,差异有统计学意义(P均<0.05)。与对照组和无义组比较,miR-125a-5p组瘤细胞增殖速度减慢,瘤细胞迁移力降低,肿瘤克隆能力减弱,荷瘤鼠肿瘤体积小,差异均有统计学意义(P<0.05)。结论 miR-125a-5P在髓母细胞瘤中表达降低或缺失,可能是潜在的抑癌基因之一。

本文引用格式

楚冬梅 , 李艳霞 , 高心静 , 刘晓智 , 严艳萍 , 刘翠苹 , 姜忠敏 , 姚玲 . miR-125a-5P 在髓母细胞瘤中的异常表达及其抑癌作用[J]. 临床儿科杂志, 2015 , 33(11) : 974 . DOI: 10.3969 j.issn.1000-3606.2015.11.014

Abstract

 Objectives To study the expression characteristics of miR-125a-5P in medulloblastoma, and its role as tumor suppressor. Methods The miR-125a-5p level was detected by stem loop Real-time RT-PCR in 58 cases of medulloblastoma tissues and 2 medulloblastoma cell lines, D341 and Daoy cells. miR-125a-5P oligonucleotide was transfected to D341 cells, then Ki-67 expression was detected by immunofluorescence assay, cell migration was tested by transwell assay, and tumorigenicity was determined by clone formation assay. The inhibitory effect of miR-125a-5p on medulloblastoma cells in vivo was detected in tumor bearing mice model. Results The relative expression level of miR-125a-5P in medulloblastoma tissues was significantly higher than that of normal children's brain tissue (t=17.545, P<0.01), while the expression level of miR-125a-5P in 2 medulloblastoma cells were significantly lower than other control cells (F=9.437, P<0.01). The expression level of miR-125a-5P was significantly higher than that of the neural stem cells, D341 and Daoy cells, and the difference was statistically significant (P<0.05). Compared with the control group and the non-sense group, the ability of proliferation, migration and clone of cells in miR-125a-5p group obviously declined (P <0.05), as well as the tumor volume in mice (P<0.05). Conclusions The expression of miR-125a-5P in medulloblastoma was reduced or absent, and was supposedly a tumor suppressor gene.
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