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儿童免疫性血小板减少症DNA 甲基转移酶mRNA 的表达

  • 肖爱菊 ,
  • 任瑞娟 ,
  • 王团结 ,
  • 曹利佳 ,
  • 石太新 ,
  • 赵东菊 ,
  • 李培岭
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  • 1. 新乡医学院第一附属医院儿内一科( 河南卫辉 453100);2. 安阳市妇幼保健院儿科( 河南安阳 455000)

收稿日期: 2015-02-15

  网络出版日期: 2015-02-15

Expression of DNA methyltransferase mRNA in children with immune thrombocytopenia

  •  XIAO Aiju ,
  • REN Ruijuan ,
  • WANG Tuanjie ,
  • CAO Lijia ,
  • SHI Taixin ,
  • ZHAO Dongju ,
  • LI Peiling
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  • 1. Department of Pediatrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China; 2. Department of Pediatrics, Maternal and Child Health Hospital of Anyang City, Anyang 455000, Henan, China

Received date: 2015-02-15

  Online published: 2015-02-15

摘要

目的 通过检测免疫性血小板减少症(ITP)患儿外周血淋巴细胞中DNA甲基转移酶1(Dnmt1)、DNA甲基转移酶3a(Dnmt3a)mRNA的表达,探讨DNA甲基化与儿童ITP发病机制之间的联系。方法 采用RT-PCR方法检测36例新诊断ITP患儿与26例健康体检儿童外周血淋巴细胞中Dnmt1、Dnmt3a mRNA的表达水平;并进行分析比较。结果 新诊断ITP患儿外周血淋巴细胞中Dnmt1的mRNA表达为(3.02±0.49),较对照组(4.58±0.52)明显降低,差异有统计学意义(t=11.95,P<0.01);ITP患儿Dnmt3a mRNA的表达为(1.49±0.44),较对照组(2.41±0.32)明显降低,差异有统计学意义(t=9.12,P<0.01)。结论 新诊断ITP患儿可能存在DNA低甲基化,这种DNA低甲基化与儿童ITP发病机制关系密切。

本文引用格式

肖爱菊 , 任瑞娟 , 王团结 , 曹利佳 , 石太新 , 赵东菊 , 李培岭 . 儿童免疫性血小板减少症DNA 甲基转移酶mRNA 的表达[J]. 临床儿科杂志, 2015 , 33(2) : 147 . DOI: 10.3969 j.issn.1000-3606.2015.02.012

Abstract

Objective To study the relationship between DNA methylation and pathogenesis of childhood immune thrombocytopenic purpura (ITP) by examining the expression of DNA methyltransferase 1(Dnmt1) and DNA methyltransferase 3a (Dnmt3a) mRNA in peripheral blood lymphocytes of the children with ITP. Methods Expression of Dnmt 1 and Dnmt3a mRNA in the peripheral blood lymphocytes in 36 children with newly diagnosed ITP and 26 healthy children were detected using RT-PCR. Results Dnmt1 mRNA expression in peripheral blood lymphocytes in children diagnosed with ITP was 3.02±0.49, significantly lower than 4.58±0.52 in the control group (t=11.95, p<0.001). Dnmt3a mRNA expression in peripheral blood lymphocytes in children diagnosed with ITP was 1.49±0.44, significantly lower than 2.41±0.32 in the control group (t=9.12, p<0.001). Conclusions Children with newly diagnosed ITP have lower DNA methylation status in peripheral blood lymphocytes as compared to that in healthy children. The DNA methylation may play an important role in the etiology of acute ITP in children.
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