Abstract: 　Objective　To report the result of annual monitoring and analysis of nasopharyngeal virus in children with respiratory tract infections in Nanxiang, Shanghai District. Methods Nasopharyngeal secretions were collected from 4389 children with acute respiratory tract infection in outpatient department from January 2007 to September 2013, 9 common respiratory viruses were analyzed by Multiplex RT-PCR, including influenza virus (FLU), parainfluenza virus (PIV), respiratory syncytical virus (RSV) , adenovirus (ADV), human bocavirus（HBOV）, human coronavirus（Cov）, enterovirus（EV）, human metapneumovirus（HMPV）, and rhinovirus（HRV）. The same analysis was done in 123 asymptomatic children during the same period. Results The positive rate of detected respiratory viruses in children with respiratory tract infections in nasopharyngeal secretions were 34.8% (1526/4389), including FLU 10.3% (453/4389), RSV 7.3% (320/4389), PIV 6.2% (274/4389), ADV 3.3% (146/4389), HBOV 2.7% (118/4389), EV 2.5% (110/4389), Cov 2.4% (105/4389), HRV 1.6% (72/4389), HMPV 1.5% (67/4389); two and more combined respiratory viral infection were found in 273 cases (6.2%). The virus detection rate between age groups was significantly different (χ2 = 41.91, P <0.001). The school-age group had the lowest positive rate of 23.4% and the positive rates in other three groups were all higher than 35.0%. The infant group had the higher positive rate of RSV and HRV. FLU detection rate in school-age group was 13.6%. Respiratory viruses in children with asthmatic disease has high detection rate. RSV infection rate was the highest 14.8% (30/204) in the asthmatic disease group, followed by HBOV 13.8% (28/204). In nasopharyngeal secretions of 123 asymptomatic children, virus-positive detection rate of 6.5% (8/123), which showed significant difference from that in respiratory virus infection group (χ2 = 42.60, P <0.001). Conclusions In seven consecutive years of testing, the influenza virus and respiratory syncytial virus play an important role in children with respiratory tract infections in this region. The detection rate of virus showed difference between different age groups and a higher detection rate of RSV in infants with respiratory tract infections was observed. The overall detection rate of virus was decreased with the increase of age excluding the influenza virus.