Abstract: 　Objectives　To explore the influence of standardized treatment and technical flow improvement on the diagnosis and treatment of congenital diaphragmatic hernia (CDH) after prenatal diagnosis. Methods　The clinical data of neonates diagnosed with CDH who were hospitalized in neonatal intensive care unit from January 2005 to August 2016 was analyzed retrospectively. Based on the start time of standardized treatment implementation, 93 cases of CDH hospitalized from January 2010 to August 2016 were divided into the intervention group while 15 cases of CDH hospitalized from January 2005 to December 2009 were divided into the control group. The survival rate and complications of clinical outcomes between two groups were compared. Results　There was no significant difference in sex, gestational age, birth weight, Apgar score, and CDH position between two groups (P ＞0.05). The total survival rate was 81.7% in the intervention group and 53.3% in the control group, and there was statistical difference (P＜0.05). The difference of mechanical ventilation mode and pneumothorax rate between two groups also had statistical difference (P ＞0.05). The median operation time in the control group was 4.5 h and the intervention group was 49.5 h. The postoperative survival rate was 61.5% in the control group and 90.5% in the intervention group, and the differences between two groups were significant (P ＞0.05). The first blood gas analysis of deaths cases in both groups showed that there were significant differences in pH and PCO2 values (P ＞0.05). Conclusions　Optimization of the clinical management during perioperative period can improve the survival rate of CDH and reduce complications. However, the dead cases in the intervention group had more severe pulmonary hypoplasia.