Abstract: Objective　To investigate the impact of an infant formula supplemented with 1.34 g/L oligosaccharides mixture (galactooligosaccharides and fructooligosaccharides, GOS+FOS) on growth, stool characteristics and fecal beneficial bacteria content in healthy infants. Methods　25 formula-fed (FF) infants and 29 breast-fed (BF) infants within 3 weeks after birth were included and followed up. FF infants were exclusively fed with GOS+FOS supplemented formula at the median age of 13d (1-27d). Body weight and head circumference at 6 and 12 weeks were measured. The frequency, consistency, and color of the infant’s stool, and occurrences of spit-up or vomiting during the study period were recorded daily by the parents. Stool samples were collected at 6 and 12 weeks of age, and then subjected to quantitative real-time polymerase chain reaction assays for the enumeration of lactobacillus, bifidobacteria, Bifidobacterium longum, Bifidobacterium breve and Bifidobacterium adolescentis. Results　No difference in daily weight gain was detected between the two groups (33.56 ± 7.03g vs. 31.18 ± 6.91g, P=0.22) during the observation phase from 6 to 12 weeks. FF infants had less frequent and firmer stools at 6 weeks than BF infants, and they both had similar stool frequency, color and consistency at the end of study. FF infants had less fecal Bifidobacterium, B. longum and B. breve at 6 weeks, but those differences became less significant at 12 weeks with more increase in fecal B. longum and B. breve levels in FF infants during the follow up. Conclusions　The GOS+FOS supplemented formula provides adequate nutrition for growth, and promotes soft stools and intestinal bifidogenic effects in healthy infants.