Abstract: Objective　To explore the genetic phenotypes of Salmonella isolates in clinical specimens of children with diarrhea and to clarify the drug resistance characteristics of Salmonella. Method　Stool specimens were collected in diarrhea children from July 2014 to October 2015, and 43 strains of Salmonella were isolated. The target gene of 43 Salmonella strains was amplified and sequenced by multilocus sequence typing (MLST). The genetic clustering analysis was performed by eBURST method, and the genetic evolution of bacterial genes was analyzed by STAT2 software. The resistance of Salmonella strains to the commonly used antibiotics was tested. Results　MLST analysis showed that 43 strains of Salmonella could be divided into 17 sequence types (ST) and no new type was found, among which ST11 and ST34 were the dominant ST types, accounting for 32.56% and 23.26% respectively. The rates of 43 Salmonella strains resistant to amoxicillin/clavulanic acid, cefotaxime, ceftazidime and cefepime were 13.95%, 27.91%, 16.28% and 11.63% respectively. The strains resistant to piperacillin/tazobactam and imipenem were not found. Conclusion　ST11 and ST34 were the dominant clones of Salmonella strains isolated from these diarrhea children, which was resistant to three- or four- generation cephalosporins, while they were still sensitive to piperacillin/ tazobactam or imipenem.