Abstract: 　Objective　To explore the clinical characteristics of Escherichia coli and Enterococcus infection in urinary tract in children. Method 　The clinical data of 117 cases of urinary tract infection with positive urine culture between 2013 and 2014 were retrospectively analyzed. Results　Among 117 children with positive urine culture, there were 55 (47%) cases of Escherichia coli and 28 (23.93%) cases of Enterococcus. The percentage of children having significant clinical manifestations in Escherichia coli group was significantly higher than that in Enterococcus group (67.27% vs 42.86%, P<0.05). Compared with Escherichia coli group, Enterococcus group had longer duration of increased urinary leucocyte count, longer hospital stay, higher drug resistance rate of urine culture and higher proportion of urinary tract malformation, and the differences were statistically significant (P<0.05). Conclusion　Escherichia coli are the most common pathogenic bacteria of urinary tract infection in children, followed by Enterococcus. Enterococcus infection is seen more in children with malformation, its clinical symptoms are atypical and it is prone to drug resistance.