血管生成素-1在高氧诱导新生鼠支气管肺发育不良的表达及与肺发育的关系

Abstract

Abstract: 　Objective　To explore the expression of angiopoietin-1 (Ang-1) and lung development in neonatal rat with hyperoxia-induced bronchopulmonary dysplasia (BPD). Methods A total of forty-eight 1- to 3-day-old neonatal rats were randomly divided into hyperoxia group and control group with 24 rats in each group, fed in high concentration oxygen (≥95%) or in air respectively. At 1^st, 3^rd and 7^th day after high oxygen exposure, the histological changes in lung tissue were observed by HE stai-ning under a light microscope and the expressions of Ang-1 mRNA and its protein in lung tissue were detected by RT-PCR and Western blot. Results With extended exposure to high concentrations of oxygen, rats in hyperoxia group presented such pathologic change of lung tissue dysplasia as alveolar simplification, reduction in alveolar number and arrested pulmonary microvascular development. At 7^th day after high oxygen exposure, Ang-1 mRNA and protein expressions in hyperoxia group were (0.33±0.18) and (0.20±0.07), significantly lower than those [(0.83±0.46) and (0.57±0.44)] in control group (P<0.05). Conclusions Ang-1 plays an important regulatory role in the pulmonary vascular development and participates in the pathogenesis of BPD.

WANG Ling,LU Hui,LI Meixue . Expression of angiopoietin-1 and lung development in neonatal rat with hyperoxia-induced BPD [J]., 2014, 32(4): 355-359.

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Expression of angiopoietin-1 and lung development in neonatal rat with hyperoxia-induced BPD

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