Abstract: 　Objective　To investigate the clinical effect of linezolid on severe Gram-positive bacteria infection in children. Methods The retrospective analysis was performed in the clinical cases of severe Gram-positive bacteria infection which were insensitive to drugs selected from experience but sensitive to linezolid according to sputum culture and sensitivity test. Results Twenty-four children with severe Gram-positive bacterial infections were treated with linezolid by intravenous infusion (10 mg/kg, q8h) for 10-14 days. All of them were markedly improved. Conclusions When drugs selected from experiences are ineffective, linezolid or in combination with other drugs is a good choice in the treatment of severe Gram-positive bacterial infections.