Abstract: Objective　To study the clinical manifestations and etiology of infective endocarditis(IE) in children. Methods　Clinical and etiology data from children with infective endocarditis were collected in Children’s Hospital, Zhejiang University School of Medicine between January 1, 2007 and December 31, 2014. Results　Thirty-five children with IE were diagnosed in the study, 15 were male and 20 femaleand with a mean age of (6.56±0.81) years.There were 23 patients had congenital heart disease (65.7%). Vegetations were found in 24 cases(68.6%), and long-time fever was found in 57.4% of cases. Positive results were identified in 19 patients (54.3%) and 83.3% weregram-positive bacteria, including coagulase-negative staphylococci, streptococcus viridans and enterococcus preparation.Drug sensitivity showed that all gram-positive strains were sensitive to vancomycin. Conclusion　Most of the cases with IE in children had congenital heart disease. The main causes of IE were gram-positive coccus, and vancomycin was effective antibiotics for the treatment of IE.