Abstract:

Objective To explore the level of expression, clinical significance of sB 7 -H 3 in the bronchoalveolar lavage fluid (BALF) of refractory Mycoplasma pneumoniae (MP) pneumonia (RMPP) in children and the relationship between sB 7 -H 3 and various cytokines. Methods The BALF of forty-three hospitalized children with RMPP (RMPP group) were collected for the diagnosis and treatment. Thirteen cases were lavaged only once and the other thirty cases had collected the BALF twice. The BALF of fifteen hospitalized children with bronchial foreign body were collected as control group. The expression levels of sB 7 -H 3 , IL- 1 β, IL- 2 and IL- 36 in the BALF were detected by enzyme-linked immunosorbent assay. The expression levels of sB 7 -H 3 , IL- 1 β, IL- 2 and IL- 36 in the BALF at the acute phase were compared with control group and the group after treatment. Analyzed the correlation between the level of sB 7 -H 3 and IL- 1 β, IL- 2 , IL- 36 in the BALF of RMPP children at acute stage. Results The levels of sB 7 -H 3 , IL- 1 β and IL- 36 in the BALF of the first lavage group were higher than those of single lavage group and control group (all P< 0 . 05 ). The levels of sB 7 -H 3 , IL- 1 β, IL- 2 and IL- 36 in the BALF of single lavage group were higher than those of control group (all P<0.05). The levels of sB7-H3, IL-1β, IL-2 and IL-36 in the BALF of the second lavage group were lower than those of the first lavage group (all P< 0 . 05 ).The levels of sB 7 -H 3 , IL- 2 in the BALF of the second lavage group were higher than those in the control group (both P< 0 . 05 ), but the levels of IL- 1 β, IL- 36 in the BALF showed no difference between the second lavage group and the control group (both P> 0 . 05 ). The levels of sB 7 -H 3 had positive correlation with the levels of IL- 1 β, IL- 2 and IL- 36 (all P< 0 . 001 ). Conclusions sB 7 -H 3 may control the secretion of IL- 1 β, IL- 2 and IL- 36 , and participate in immune response and lung injury after MP infection, which may lead to occurrence and development of RMPP.